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ILKAP Kit ELISA

ILKAP Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690894
  • Antigène Voir toutes ILKAP Kits ELISA
    ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))
    Reactivité
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human ILKAP ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human ILKAP.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes ILKAP Kits ELISA
    ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))
    Autre désignation
    ILKAP (ILKAP Produits)
    Synonymes
    MGC84595 Kit ELISA, MGC107938 Kit ELISA, ILKAP Kit ELISA, ILKAP2 Kit ELISA, ILKAP3 Kit ELISA, PP2C-DELTA Kit ELISA, 0710007A14Rik Kit ELISA, 1600009O09Rik Kit ELISA, ILK associated serine/threonine phosphatase Kit ELISA, ILK associated serine/threonine phosphatase L homeolog Kit ELISA, integrin-linked kinase-associated serine/threonine phosphatase Kit ELISA, integrin-linked kinase-associated serine/threonine phosphatase 2C Kit ELISA, ILKAP Kit ELISA, ilkap.L Kit ELISA, ilkap Kit ELISA, Ilkap Kit ELISA
    ID gène
    80895
    UniProt
    Q9H0C8
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