IMPDH1 Kit ELISA

N° du produit ABIN5690895

Détail du produit IMPDH1 Kit ELISA

Antigène: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human IMPDH1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human IMPDH1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: IMPDH1 (Inosine 5'-Phosphate Dehydrogenase 1 (IMPDH1))

Autre désignation: IMPDH1 (IMPDH1 Produits)

Synonymes: IMPD Kit ELISA, IMPD1 Kit ELISA, LCA11 Kit ELISA, RP10 Kit ELISA, sWSS2608 Kit ELISA, B930086D20Rik Kit ELISA, IMPD 1 Kit ELISA, IMPDH 1 Kit ELISA, D3 Kit ELISA, IMPD 1b Kit ELISA, IMPDH 1b Kit ELISA, IMPDH1 Kit ELISA, id:ibd5035 Kit ELISA, si:dkey-31f5.7 Kit ELISA, wu:fa09h11 Kit ELISA, wu:fa99c03 Kit ELISA, zgc:113446 Kit ELISA, imp Kit ELISA, imp1 Kit ELISA, imp2 Kit ELISA, IMPD 1a Kit ELISA, IMPDH 1a Kit ELISA, zgc:91911 Kit ELISA, inosine monophosphate dehydrogenase 1 Kit ELISA, inosine-5'-monophosphate dehydrogenase 1 Kit ELISA, IMP (inosine 5'-monophosphate) dehydrogenase 1b Kit ELISA, IMP (inosine 5'-monophosphate) dehydrogenase 1 Kit ELISA, IMP (inosine 5'-monophosphate) dehydrogenase 1a Kit ELISA, IMPDH1 Kit ELISA, Impdh1 Kit ELISA, LOC100442769 Kit ELISA, impdh1 Kit ELISA, impdh1b Kit ELISA, impdh1.L Kit ELISA, impdh1a Kit ELISA

ID gène: 3614

UniProt: P20839

Pathways: Ribonucleoside Biosynthetic Process