JNK2 Kit ELISA

N° du produit ABIN5690901

Détail du produit JNK2 Kit ELISA

Antigène: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Épitope: Isoform alpha-1

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human JNK2/MAPK9 isoform alpha-1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human JNK2.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: JNK2 (MAPK9) (Mitogen-Activated Protein Kinase 9 (MAPK9))

Autre désignation: JNK2 (MAPK9 (MAPK9 Produits)

Synonymes: MAPK9 Kit ELISA, JNK-55 Kit ELISA, JNK2 Kit ELISA, JNK2A Kit ELISA, JNK2ALPHA Kit ELISA, JNK2B Kit ELISA, JNK2BETA Kit ELISA, PRKM9 Kit ELISA, SAPK Kit ELISA, SAPK1a Kit ELISA, p54a Kit ELISA, p54aSAPK Kit ELISA, AI851083 Kit ELISA, Prkm9 Kit ELISA, JNK2-ALPHA1 Kit ELISA, MAP kinase 9 Kit ELISA, mitogen-activated protein kinase 9 Kit ELISA, MAP kinase 9 Kit ELISA, MAPK9 Kit ELISA, Mapk9 Kit ELISA, MPK9 Kit ELISA

Pathways: Signalisation MAPK, Signalisation WNT, Signalisation TLR, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Positive Regulation of Endopeptidase Activity, Hepatitis C, Toll-Like Receptors Cascades, BCR Signaling, S100 Proteins