Myogenin Kit ELISA

N° du produit ABIN5690915

Détail du produit Myogenin Kit ELISA

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Myogenin ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Myogenin.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Myogenin (MYOG) (Myogenin (Myogenic Factor 4) (MYOG))

Autre désignation: Myogenin (MYOG Produits)

Synonymes: cb553 Kit ELISA, zgc:100763 Kit ELISA, LOC446037 Kit ELISA, myf4 Kit ELISA, Xmyog Kit ELISA, myogenin Kit ELISA, LOC100136471 Kit ELISA, LOC100224062 Kit ELISA, LOC100303673 Kit ELISA, MYF4 Kit ELISA, bHLHc3 Kit ELISA, myo Kit ELISA, myf-4 Kit ELISA, myogenin Kit ELISA, myogenin (myogenic factor 4) Kit ELISA, MYOG Kit ELISA, myog Kit ELISA, LOC100303673 Kit ELISA, Myog Kit ELISA

ID gène: 4656

UniProt: P15173

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development