NFATC3 Kit ELISA
NFATC3
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690919
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Antigène
Voir toutes NFATC3 Kits ELISA
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NFATC3
(Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human NF-ATc3 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificité
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This ELISA antibody pair recognizes Human NF-ATc3.
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Top Product
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Discover our top product NFATC3 Kit ELISA
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Stock
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4 °C
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Date de péremption
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6 months
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Antigène
Voir toutes NFATC3 Kits ELISA
-
NFATC3
(Nuclear Factor of Activated T-Cells, Cytoplasmic, Calcineurin-Dependent 3 (NFATC3))
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Autre désignation
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NF-ATc3 (NFATC3 Produits)
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Synonymes
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NFATC3 Kit ELISA, NFATx Kit ELISA, nf-at Kit ELISA, nfat4 Kit ELISA, nfatx Kit ELISA, C80703 Kit ELISA, D8Ertd281e Kit ELISA, NFAT4 Kit ELISA, NFATX Kit ELISA, nuclear factor of activated T-cells 3 Kit ELISA, nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 3 Kit ELISA, nuclear factor of activated T-cells 3 S homeolog Kit ELISA, NFATC3 Kit ELISA, nfatc3 Kit ELISA, Nfatc3 Kit ELISA, nfatc3.S Kit ELISA
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ID gène
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4775
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UniProt
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Q12968
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Pathways
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Signalisation RTK, Signalisation WNT, Fc-epsilon Receptor Signaling Pathway, Chromatin Binding
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