BCAR1 Kit ELISA

N° du produit ABIN5690924

Détail du produit BCAR1 Kit ELISA

Antigène: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human p130Cas/BCAR1/CAS ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human p130Cas.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: BCAR1 (Breast Cancer Anti-Estrogen Resistance 1 (BCAR1))

Autre désignation: p130Cas (BCAR1/CAS) (BCAR1 Produits)

Synonymes: CAS Kit ELISA, CAS1 Kit ELISA, CASS1 Kit ELISA, CRKAS Kit ELISA, P130Cas Kit ELISA, AI385681 Kit ELISA, Cas Kit ELISA, Crkas Kit ELISA, P130CAS Kit ELISA, MGC68466 Kit ELISA, cas Kit ELISA, p130cas Kit ELISA, BCAR1 Kit ELISA, fc17f11 Kit ELISA, wu:fc17f11 Kit ELISA, zgc:175192 Kit ELISA, BCAR1, Cas family scaffolding protein Kit ELISA, breast cancer anti-estrogen resistance 1 Kit ELISA, breast cancer anti-estrogen resistance 1 L homeolog Kit ELISA, breast cancer anti-estrogen resistance protein 1 Kit ELISA, BCAR1 Kit ELISA, Bcar1 Kit ELISA, bcar1.L Kit ELISA, bcar1 Kit ELISA, LOC100435586 Kit ELISA

ID gène: 9564

UniProt: P56945

Pathways: EGFR Signaling Pathway, Neurotrophin Signaling Pathway, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling