Peroxiredoxin 5 Kit ELISA

N° du produit ABIN5690930

Détail du produit Peroxiredoxin 5 Kit ELISA

Antigène: Peroxiredoxin 5 (PRDX5)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Peroxiredoxin-5 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human Peroxiredoxin-5.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Peroxiredoxin 5 (PRDX5)

Autre désignation: Peroxiredoxin-5 (PRDX5 Produits)

Synonymes: acr1 Kit ELISA, aoeb166 Kit ELISA, pmp20 Kit ELISA, prx-5 Kit ELISA, prx5 Kit ELISA, prxv Kit ELISA, sbbi10 Kit ELISA, GB16965 Kit ELISA, zgc:112318 Kit ELISA, PRDX5 Kit ELISA, CG 32920 Kit ELISA, CG 7217 Kit ELISA, CG32920 Kit ELISA, CG7217 Kit ELISA, Dmel\\CG7217 Kit ELISA, dPrx5 Kit ELISA, dprx5 Kit ELISA, ACR1 Kit ELISA, AOEB166 Kit ELISA, B166 Kit ELISA, PLP Kit ELISA, PMP20 Kit ELISA, PRDX6 Kit ELISA, PRXV Kit ELISA, prx-V Kit ELISA, AOPP Kit ELISA, Pmp20 Kit ELISA, Prdx6 Kit ELISA, PrxV Kit ELISA, Aoeb166 Kit ELISA, Prx V Kit ELISA, peroxiredoxin 5 L homeolog Kit ELISA, peroxiredoxin-5, mitochondrial Kit ELISA, peroxiredoxin 5 Kit ELISA, Peroxiredoxin 5 Kit ELISA, prdx5.L Kit ELISA, LOC552429 Kit ELISA, prdx5 Kit ELISA, PRDX5 Kit ELISA, Prx5 Kit ELISA, Prdx5 Kit ELISA

ID gène: 25824

UniProt: P30044

Pathways: Cell RedoxHomeostasis