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PGPEP1 Kit ELISA

PGPEP1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690932
  • Antigène Voir toutes PGPEP1 Kits ELISA
    PGPEP1 (Pyroglutamyl-Peptidase I (PGPEP1))
    Reactivité
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human PGPEP-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human PGPEP-1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes PGPEP1 Kits ELISA
    PGPEP1 (Pyroglutamyl-Peptidase I (PGPEP1))
    Autre désignation
    PGPEP-1 (PGPEP1 Produits)
    Synonymes
    PGP Kit ELISA, PGP-I Kit ELISA, PGPI Kit ELISA, Pcp Kit ELISA, 2810003H13Rik Kit ELISA, Gm19458 Kit ELISA, PAP-I Kit ELISA, PAPI Kit ELISA, pyroglutamyl-peptidase I Kit ELISA, Pyroglutamyl-peptidase I Kit ELISA, Fnod_0998 Kit ELISA, Tlet_1256 Kit ELISA, Franean1_3440 Kit ELISA, Sked_37410 Kit ELISA, Arch_1014 Kit ELISA, Olsu_1567 Kit ELISA, Bcell_2984 Kit ELISA, Deima_2337 Kit ELISA, Spico_0458 Kit ELISA, Poras_1572 Kit ELISA, PGPEP1 Kit ELISA, Pgpep1 Kit ELISA
    ID gène
    54858
    UniProt
    Q9NXJ5
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