SEMA3E Kit ELISA
SEMA3E
Reactivité: Humain
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690947
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Antigène
Voir toutes SEMA3E Kits ELISA
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SEMA3E
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))
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Reactivité
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Humain
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Human Semaphorin-3E ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificité
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This ELISA antibody pair recognizes Human Semaphorin-3E.
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Stock
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4 °C
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Date de péremption
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6 months
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Antigène
Voir toutes SEMA3E Kits ELISA
-
SEMA3E
(Sema Domain, Immunoglobulin Domain (Ig), Short Basic Domain, Secreted, (Semaphorin) 3E (SEMA3E))
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Autre désignation
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Semaphorin-3E (SEMA3E Produits)
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Synonymes
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M-SEMAH Kit ELISA, M-SemaK Kit ELISA, SEMAH Kit ELISA, coll-5 Kit ELISA, 6430702L12 Kit ELISA, AA408817 Kit ELISA, Semah Kit ELISA, mKIAA0331 Kit ELISA, semaphorin 3E Kit ELISA, sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E Kit ELISA, SEMA3E Kit ELISA, Sema3e Kit ELISA
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ID gène
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9723
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UniProt
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O15041
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