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SGK1 Kit ELISA

SGK1 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690949
  • Antigène Voir toutes SGK1 Kits ELISA
    SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
    Reactivité
    • 4
    • 2
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human SGK1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human SGK1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes SGK1 Kits ELISA
    SGK1 (serum/glucocorticoid Regulated Kinase 1 (SGK1))
    Autre désignation
    SGK1 (SGK1 Produits)
    Synonymes
    SGK Kit ELISA, Sgk Kit ELISA, sgk-B Kit ELISA, sgk2 Kit ELISA, cb1083 Kit ELISA, sgk Kit ELISA, wu:fc20a09 Kit ELISA, sgk-A Kit ELISA, sgk1 Kit ELISA, sgk1-a Kit ELISA, sgk1-b Kit ELISA, serum/glucocorticoid regulated kinase 1 Kit ELISA, Serine/threonine-protein kinase sgk-1 Kit ELISA, serum/glucocorticoid regulated kinase 1 S homeolog Kit ELISA, serum/glucocorticoid regulated kinase 1 L homeolog Kit ELISA, SGK1 Kit ELISA, Sgk1 Kit ELISA, sgk-1 Kit ELISA, sgk1.S Kit ELISA, sgk1 Kit ELISA, sgk1.L Kit ELISA
    ID gène
    6446
    UniProt
    O00141
    Pathways
    Signalisation MAPK, Signalisation Notch, Steroid Hormone Mediated Signaling Pathway
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