TSC1 Kit ELISA

N° du produit ABIN5690964

Détail du produit TSC1 Kit ELISA

Antigène: TSC1 (Tuberous Sclerosis 1 (TSC1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human TSC1/Hamartin ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human TSC1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: TSC1 (Tuberous Sclerosis 1 (TSC1))

Autre désignation: TSC1 (Hamartin (TSC1 Produits)

Synonymes: LAM Kit ELISA, TSC Kit ELISA, hamartin Kit ELISA, mKIAA0243 Kit ELISA, tsc1 Kit ELISA, wu:fc38b04 Kit ELISA, fa99f04 Kit ELISA, wu:fa99f04 Kit ELISA, zgc:63657 Kit ELISA, TSC complex subunit 1 Kit ELISA, tuberous sclerosis 1 Kit ELISA, TSC complex subunit 1b Kit ELISA, TSC complex subunit 1a Kit ELISA, TSC1 Kit ELISA, Tsc1 Kit ELISA, tsc1b Kit ELISA, tsc1 Kit ELISA, tsc1a Kit ELISA

ID gène: 7248

UniProt: Q92574

Pathways: Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Tube Formation