Mps1 Kit ELISA

N° du produit ABIN5690965

Détail du produit Mps1 Kit ELISA

Antigène: Mps1 (TTK)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human TTK/PYT ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human TTK.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: Mps1 (TTK)

Autre désignation: TTK (PYT (TTK Produits)

Synonymes: CT96 Kit ELISA, ESK Kit ELISA, MPH1 Kit ELISA, MPS1 Kit ELISA, MPS1L1 Kit ELISA, PYT Kit ELISA, AL022661 Kit ELISA, Esk1 Kit ELISA, Mps1 Kit ELISA, esk Kit ELISA, mps1 Kit ELISA, ncp Kit ELISA, Ttk protein kinase Kit ELISA, TTK protein kinase Kit ELISA, ttk protein kinase Kit ELISA, Ttk Kit ELISA, TTK Kit ELISA, ttk Kit ELISA

ID gène: 7272

UniProt: P33981