UFM1 Kit ELISA

N° du produit ABIN5690967

Détail du produit UFM1 Kit ELISA

Antigène: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human UFM1 ELISA Kit.

Type d'échantillon: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Specificité: This ELISA antibody pair recognizes Human UFM1.

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 6 months

Détail du antigène

Antigène: UFM1 (Ubiquitin-Fold Modifier 1 (UFM1))

Autre désignation: UFM1 (UFM1 Produits)

Synonymes: T32E8.4 Kit ELISA, T32E8_4 Kit ELISA, BM-002 Kit ELISA, C13orf20 Kit ELISA, bA131P10.1 Kit ELISA, 1810045K17Rik Kit ELISA, AI132708 Kit ELISA, AI463323 Kit ELISA, ENSMUSG00000074598 Kit ELISA, Gm10726 Kit ELISA, RGD1304890 Kit ELISA, UBFM1 Kit ELISA, ubfm1 Kit ELISA, wu:fa10f05 Kit ELISA, wu:fb72g01 Kit ELISA, zgc:55335 Kit ELISA, ubiquitin fold modifier 1 Kit ELISA, ubiquitin-fold modifier 1 Kit ELISA, ubiquitin-fold modifier Kit ELISA, ubiquitin-fold modifier 1 L homeolog Kit ELISA, UFM1 Kit ELISA, ufm1 Kit ELISA, CCP2 Kit ELISA, Ufm1 Kit ELISA, ufm1.L Kit ELISA, PR46a Kit ELISA

ID gène: 51569

UniProt: P61960