CILP Kit ELISA
CILP
Reactivité: Porc
Colorimetric
Sandwich ELISA
Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690987
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Antigène
Voir toutes CILP Kits ELISA
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CILP
(Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))
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Reactivité
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Porc
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Méthode de détection
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Colorimetric
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Type de méthode
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Sandwich ELISA
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Application
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ELISA
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Fonction
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Porcine (pig) CILP-1 ELISA Kit.
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Type d'échantillon
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Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Analytical Method
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Quantitative
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Specificité
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This ELISA antibody pair recognizes Porcine (pig) CILP-1.
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Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
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Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
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Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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Plaque
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Pre-coated
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Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
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Restrictions
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For Research Use only
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Stock
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4 °C
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Date de péremption
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6 months
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Antigène
Voir toutes CILP Kits ELISA
-
CILP
(Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))
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Autre désignation
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CILP-1 (CILP Produits)
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Synonymes
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CILP-1 Kit ELISA, HsT18872 Kit ELISA, 9830114D16 Kit ELISA, C130036G17Rik Kit ELISA, NTPPH Kit ELISA, CILP Kit ELISA, im:7137994 Kit ELISA, cartilage intermediate layer protein Kit ELISA, cartilage intermediate layer protein, nucleotide pyrophosphohydrolase Kit ELISA, cartilage intermediate layer protein 1 Kit ELISA, CILP Kit ELISA, Cilp Kit ELISA, cilp Kit ELISA, LOC100336614 Kit ELISA
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UniProt
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O19112
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