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CILP Kit ELISA

CILP Reactivité: Porc Colorimetric Sandwich ELISA Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
N° du produit ABIN5690987
  • Antigène Voir toutes CILP Kits ELISA
    CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))
    Reactivité
    • 3
    • 1
    • 1
    Porc
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Porcine (pig) CILP-1 ELISA Kit.
    Type d'échantillon
    Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Porcine (pig) CILP-1.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    4 °C
    Date de péremption
    6 months
  • Antigène Voir toutes CILP Kits ELISA
    CILP (Cartilage Intermediate Layer Protein, Nucleotide Pyrophosphohydrolase (CILP))
    Autre désignation
    CILP-1 (CILP Produits)
    Synonymes
    CILP-1 Kit ELISA, HsT18872 Kit ELISA, 9830114D16 Kit ELISA, C130036G17Rik Kit ELISA, NTPPH Kit ELISA, CILP Kit ELISA, im:7137994 Kit ELISA, cartilage intermediate layer protein Kit ELISA, cartilage intermediate layer protein, nucleotide pyrophosphohydrolase Kit ELISA, cartilage intermediate layer protein 1 Kit ELISA, CILP Kit ELISA, Cilp Kit ELISA, cilp Kit ELISA, LOC100336614 Kit ELISA
    UniProt
    O19112
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