ENPP2 Kit ELISA

N° du produit ABIN5692213

Détail du produit ENPP2 Kit ELISA

Antigène: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Épitope: AA 36-863

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 625 pg/mL - 40000 pg/mL

Seuil minimal de détection: 625 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Autotaxin. 96wells/kit, with removable strips.

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Milk, Plasma (heparin), Serum, Urine

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO, Immunogen sequence: A36-I863

Sensibilité: < 10 pg/mL

Ingrédients: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Information d'application

Durée du test: 15 min

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Date de péremption: 12 months

Détail du antigène

Antigène: ENPP2 (Ectonucleotide Pyrophosphatase/phosphodiesterase 2 (ENPP2))

Autre désignation: ENPP2 (ENPP2 Produits)

Synonymes: ATX Kit ELISA, ATX-X Kit ELISA, AUTOTAXIN Kit ELISA, LysoPLD Kit ELISA, NPP2 Kit ELISA, PD-IALPHA Kit ELISA, PDNP2 Kit ELISA, Npps2 Kit ELISA, PD-Ialpha Kit ELISA, Pdnp2 Kit ELISA, MGC132047 Kit ELISA, atx Kit ELISA, zgc:63550 Kit ELISA, autotaxin Kit ELISA, enpp2 Kit ELISA, enpp2a Kit ELISA, enpp2b Kit ELISA, ectonucleotide pyrophosphatase/phosphodiesterase 2 Kit ELISA, ectonucleotide pyrophosphatase/phosphodiesterase 2 L homeolog Kit ELISA, ectonucleotide pyrophosphatase/phosphodiesterase 2 S homeolog Kit ELISA, ENPP2 Kit ELISA, Enpp2 Kit ELISA, enpp2 Kit ELISA, enpp2.L Kit ELISA, enpp2.S Kit ELISA

Sujet:

Synonyms: Ectonucleotide pyrophosphatase/phosphodiesterase family member 2, E-NPP 2, Autotaxin, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2

Tissue Specificity: Predominantly expressed in brain, placenta, ovary, and small intestine. Expressed in a number of carcinomas such as hepatocellular and prostate carcinoma, neuroblastoma and non-small-cell lung cancer. Expressed in body fluids such as plasma, cerebral spinal fluid (CSF), saliva, follicular and amniotic fluids. Not detected in leukocytes. Isoform 1 is more highly expressed in peripheral tissues than in the central nervous system (CNS). Adipocytes only express isoform 1. Isoform 3 is more highly expressed in the brain than in peripheral tissues.

Background: Autotaxin, also known as ectonucleotide pyrophosphatase/phosphodiesterase family member 2 (E-NPP 2), is an enzyme that in humans is encoded by the ENPP2 gene. The protein encoded by this gene functions as both a phosphodiesterase, which cleaves phosphodiester bonds at the 5' end of oligonucleotides, and a phospholipase, which catalyzes production of lysophosphatidic acid (LPA) in extracellular fluids. LPA evokes growth factor-like responses including stimulation of cell proliferation and chemotaxis. This gene product stimulates the motility of tumor cells and has angiogenic properties, and its expression is upregulated in several kinds of carcinomas. The gene product is secreted and further processed to make the biologically active form. Several alternatively spliced transcript variants encoding different isoforms have been identified.

Cellular Localisation: Secreted.

UniProt: Q13822