DKK4 Kit ELISA

N° du produit ABIN5692231

Détail du produit DKK4 Kit ELISA

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Épitope: AA 19-224

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human DKK-4. 96wells/kit, with removable strips.

Marque: PicoKine™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificité: Expression system for standard: NSO, Immunogen sequence: L19-L224

Sensibilité: < 10 pg/mL

Ingrédients: 96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
Adhesive cover

Information d'application

Durée du test: 15 min

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)

Date de péremption: 12 months

Détail du antigène

Antigène: DKK4 (Dickkopf Homolog 4 (Xenopus Laevis) (DKK4))

Autre désignation: DKK4 (DKK4 Produits)

Synonymes: DKK-4 Kit ELISA, Dkk-4 Kit ELISA, RGD1563172 Kit ELISA, dickkopf WNT signaling pathway inhibitor 4 Kit ELISA, DKK4 Kit ELISA, Dkk4 Kit ELISA

Sujet:

Synonyms: Dickkopf-related protein 4, Dickkopf-4, Dkk-4, hDkk-4, Dickkopf-related protein 4 short form, DKK4

Tissue Specificity: Expressed in cerebellum, T-cells, esophagus and lung.

Background: DKK4 is a membersof the Dickkopf family which are negative regulators of the growth-promoting Wnt / beta-catenin signaling pathway. By FISH, the DKK4 gene was mapped to chromosome 8p11.2-p11.1. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. Activity of this protein is modulated by binding to the Wnt co-receptor and the co-factor kremen 2. The expression of DKK4 was significantly reduced in hepatocellular carcinomas (HCCs), particularly in HCCs that showed cytoplasmic beta- catenin accumulation. DKK4 expression was also reduced in HCC cell lines. Ectopic expression of DKK4 in 2 HCC cell lines reduced beta-catenin and cyclin D1 protein levels and attenuated beta-catenin activity in reporter gene assays.

Cellular Localisation: Secreted.

UniProt: Q9UBT3

Pathways: Signalisation WNT