IL12A Kit ELISA
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- Antigène Voir toutes IL12A Kits ELISA
- IL12A (Interleukin 12 alpha (IL12A))
- Épitope
- AA 23-215, AA 23-335
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Reactivité
- Rat
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 15.6 pg/mL - 1000 pg/mL
- Seuil minimal de détection
- 15.6 pg/mL
- Application
- ELISA
- Fonction
- Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IL-12(P70). 96wells/kit, with removable strips.
- Marque
- PicoKine™
- Type d'échantillon
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- Expression system for standard: sf21, Immunogen sequence: (M23-S335)+(R23-S215)
- Sensibilité
- < 1.5 pg/mL
- Ingrédients
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96-well plate precoated with antibody
lyophilized recombinant standard
biotinylated antibody (dilution 1:100)
Avidin-Biotin-Peroxidase Complex(ABC)(dilution 1:100)
Sample diluent buffer
Antibody diluent buffer
ABC diluent buffer
TMB color developing agent
TMB stop solution
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- Durée du test
- 15 min
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)
- Date de péremption
- 12 months
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- Antigène Voir toutes IL12A Kits ELISA
- IL12A (Interleukin 12 alpha (IL12A))
- Autre désignation
- Il12a (IL12A Produits)
- Sujet
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Synonyms: Interleukin-12 subunit alpha, IL-12A, Cytotoxic lymphocyte maturation factor 35 kDa subunit, CLMF p35, IL-12 subunit p35, Il12a
Background: Interleukin (IL)-12 is a 70-KDa cytokine comprised of two disulfide-linked proteins (p35 and p40) and is essential for the initiation of effective immune response. And the IL-12p70 is a heterodimer of p35 and p40 subunits, it is an important cytokine secreted by antigen-presenting cells in response to antigenic stimulation. Gene expression analysis of the IL-12 cytokine family subunits revealed that both strains induced high levels of p40 (protein chain communal to IL-12 p70 and IL-23) as well as p19, a subunit of IL-23. Conversely only ACT- 18HS19 infection induced consistent transcription of IL-12 p35, a subunit of IL-12 p70. The standard product used in this kit is recombinant rat IL-12 p70 comprising of a 193 amino acids p35 chain and a 313 amino acids p40 chain connected by a disulfide bond.
Cellular Localisation: Secreted. - UniProt
- Q9R103
- Pathways
- Signalistation JAK/STAT, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation
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