MYOD1 Kit ELISA

N° du produit ABIN5706920

Détail du produit MYOD1 Kit ELISA

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78.1 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78.1 pg/mL

Application: ELISA

Type d'échantillon: Plasma, Serum, Tissue Homogenate

Analytical Method: Quantitative

Sensibilité: 29 pg/mL

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Commentaires:

Pre-coated, ready to use 96-well strip plate,
Standard (freeze dried),
Standard Diluent,
Detection Reagent A,
Detection Reagent B,
Assay Diluent A,
Assay Diluent B,
TMB substrate,
Stop Solution,
Wash Buffer(30 x concenthumane),
Plate sealer for 96 wells,
Instruction manual

Volume d'échantillon: 100 µL

Durée du test: 1 - 4.5 h

Plaque: Pre-coated

Protocole: 1. Prepare all reagents, samples and standards
2. Add 100µL standard or sample to each well. Incubate 2 hours at 37°C
3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C
4. Aspirate and wash 3 times
5. Add 100µL prepared Detection Reagent B. Incubate 1 hour at 37°C
6. Aspirate and wash 5 times
7. Add 90µL Substrate Solution. Incubate 15-25 minutes at 37°C
8. Add 50µL Stop Solution. Read at 450nm immediately.

Restrictions: For Research Use only

Stockage

Stock: 4 °C

Date de péremption: 12 months

Détail du antigène

Antigène: MYOD1 (Myogenic Differentiation 1 (MYOD1))

Autre désignation: Myogenic Differentiation (MyoD) (MYOD1 Produits)

Sujet: Alternative name: MYOD1, PUM, MYF3, bHLHc1, Myoblast Determination Protein 1, Myogenic Factor 3, Class C basic helix-loop-helix protein 1

Pathways: Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development