Estrogen Receptor alpha Kit ELISA

N° du produit ABIN6244373

Détail du produit Estrogen Receptor alpha Kit ELISA

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Épitope: pSer118, total

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Phospho-Estrogen receptor (Ser118) and Total Estrogen receptor ELISA Kit. This assay semi-quantitatively measures Estrogen receptor phosphorylated at Serine-118 as well as total Estrogen receptor in cell lysate samples.

Type d'échantillon: Cell Samples, Tissue Lysate

Analytical Method: Semi-Quantitative

Specificité: This ELISA kit recognizes Human Estrogen receptor phosphorylated at site Serine-118 as well as total Estrogen receptor .

Attributs du produit:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Anti-Phospho Antibody
• Anti-Pan Antibody
• HRP-Conjugated Secondary Antibody
• Streptavidin-Conjugated HRP
• Assay Diluent
• TMB One-Step Substrate
• Stop Solution
• Lysis Buffer
• Positive Control Sample

Matériel non inclus:

• Distilled or deionized water
• 100 mL and 1 liter graduated cylinders
• Tubes to prepare sample dilutions
• Protease and Phosphatase inhibitors
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• Benchtop rocker or shaker
• Microplate reader capable of measuring absorbance at 450 nm

Information d'application

Indications d'application: Optimal working dilution should be determined by the investigator.

Plaque: Pre-coated

Protocole:

1. Prepare all reagents and samples as instructed in the manual.
2. Add 100 μL of sample or positive control to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared primary antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared 1X HRP-Streptavidin to each well.
7. Incubate 1 h at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: Upon receipt, the kit should be stored at -20 °C. Please use within 6 months from the date of shipment. After initial use, Wash Buffer Concentrate (Item B), Assay Diluent (Item E), TMB One-Step Substrate Reagent (Item H), HRP-Streptavidin (Item G), Stop Solution (Item I) and Cell Lysate Buffer (Item J) should be stored at 4 °C to avoid repeated freeze-thaw cycles. Return unused wells to the pouch containing desiccant pack, reseal along entire edge and store at -20 °C. Reconstituted Positive Control (Item K) should be stored at -70 °C.

Date de péremption: 6 months

Détail du antigène

Antigène: Estrogen Receptor alpha (ESR1) (Estrogen Receptor 1 (ESR1))

Autre désignation: Estrogen receptor (ESR1 Produits)

Synonymes: LOC398734 Kit ELISA, AA420328 Kit ELISA, AU041214 Kit ELISA, ER-alpha Kit ELISA, ER[a] Kit ELISA, ERa Kit ELISA, ERalpha Kit ELISA, ESR Kit ELISA, Estr Kit ELISA, Estra Kit ELISA, Nr3a1 Kit ELISA, NR3A1 Kit ELISA, eralpha Kit ELISA, zfER[a] Kit ELISA, ER Kit ELISA, ESRA Kit ELISA, ESTRR Kit ELISA, Era Kit ELISA, akap12 Kit ELISA, cb27 Kit ELISA, esr1 Kit ELISA, fb72g12 Kit ELISA, id:ibd1202 Kit ELISA, sb:cb27 Kit ELISA, si:ch73-192g21.1 Kit ELISA, wu:fb72g12 Kit ELISA, wu:fc21c03 Kit ELISA, Esr Kit ELISA, RNESTROR Kit ELISA, ERALPHA Kit ELISA, er Kit ELISA, esr Kit ELISA, nr3a1 Kit ELISA, XER Kit ELISA, era Kit ELISA, esra Kit ELISA, ERbeta Kit ELISA, xesr-1 Kit ELISA, xlERalpha1 Kit ELISA, xlERalpha2 Kit ELISA, ESR1 Kit ELISA, estrogen receptor 1 L homeolog Kit ELISA, estrogen receptor 1 (alpha) Kit ELISA, estrogen receptor 1 Kit ELISA, A kinase (PRKA) anchor protein 12b Kit ELISA, esr1.L Kit ELISA, Esr1 Kit ELISA, ESR1 Kit ELISA, esr1 Kit ELISA, akap12b Kit ELISA

ID gène: 2099

UniProt: P03372

Pathways: Nuclear Receptor Transcription Pathway, EGFR Signaling Pathway, Retinoic Acid Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Steroid Hormone Mediated Signaling Pathway, Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly