IL1A Kit ELISA
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- Antigène Voir toutes IL1A Kits ELISA
- IL1A (Interleukin 1 alpha (IL1A))
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 0.5-300 pg/mL
- Seuil minimal de détection
- 0.5 pg/mL
- Application
- ELISA
- Fonction
- Human IL-1 alpha (IL-1 F1) ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-10, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin, MCP- 1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- Sensibilité
- 0.5 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Discover our best selling IL1A Kit ELISA
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- Discover our top product IL1A Kit ELISA
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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-
Topical Administration of Lactiplantibacillus plantarum (SkinDuoTM) Serum Improves Anti-Acne Properties." dans: Microorganisms, Vol. 11, Issue 2, (2023) (PubMed).
: "Effect of cadmium on the expression levels of interleukin-1α and interleukin-10 cytokines in human lung cells." dans: Molecular medicine reports, Vol. 12, Issue 5, pp. 6422-6, (2015) (PubMed).
: "Malignant small bowel obstruction: the last frontier for gastrointestinal stenting." dans: Journal of gastroenterology and hepatology, Vol. 27, Issue 7, pp. 1136-7, (2012) (PubMed).
: "Evaluating ESWL-induced renal injury based on urinary TNF-α, IL-1α, and IL-6 levels." dans: Urological research, Vol. 40, Issue 5, pp. 569-73, (2012) (PubMed).
: "The protective effects of coumestrol against amyloid-β peptide- and lipopolysaccharide-induced toxicity on mice astrocytes." dans: Neurological research, Vol. 33, Issue 6, pp. 663-72, (2012) (PubMed).
: "Bystander effect in tumor cells produced by Iodine-125 labeled human lymphocytes." dans: International journal of radiation biology, Vol. 88, Issue 12, pp. 1019-27, (2012) (PubMed).
: "Tumor necrosis factor-alpha (TNF-α)-blockade-induced hepatic sarcoidosis in psoriatic arthritis (PsA): case report and review of the literature." dans: Clinical rheumatology, Vol. 30, Issue 1, pp. 133-7, (2011) (PubMed).
: "Modeled repetitive motion strain and indirect osteopathic manipulative techniques in regulation of human fibroblast proliferation and interleukin secretion." dans: The Journal of the American Osteopathic Association, Vol. 107, Issue 12, pp. 527-36, (2008) (PubMed).
: "
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Topical Administration of Lactiplantibacillus plantarum (SkinDuoTM) Serum Improves Anti-Acne Properties." dans: Microorganisms, Vol. 11, Issue 2, (2023) (PubMed).
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- Antigène Voir toutes IL1A Kits ELISA
- IL1A (Interleukin 1 alpha (IL1A))
- Autre désignation
- IL-1 alpha (IL1A Produits)
- Synonymes
- IL-1A Kit ELISA, IL1 Kit ELISA, IL1-ALPHA Kit ELISA, IL1F1 Kit ELISA, IL-6 Kit ELISA, Il-1a Kit ELISA, IL-1 alpha Kit ELISA, IL-1alpha Kit ELISA, L1A Kit ELISA, interleukin 1 alpha Kit ELISA, interleukin 6 Kit ELISA, IL1A Kit ELISA, IL6 Kit ELISA, Il1a Kit ELISA
- Sujet
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Gene Names: IL1A IL1F1
Protein names: Interleukin-1 alpha (IL-1 alpha) (Hematopoietin-1)
- ID gène
- 3552
- UniProt
- P01583
- Pathways
- Signalisation NF-kappaB, Autophagy, Cancer Immune Checkpoints
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