KIT Ligand Kit ELISA
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- Antigène Voir toutes KIT Ligand (KITLG) Kits ELISA
- KIT Ligand (KITLG)
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Reactivité
- Humain
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 2-2000 pg/mL
- Seuil minimal de détection
- 2 pg/mL
- Application
- ELISA
- Fonction
- Human SCF ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Plasma, Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
- This ELISA kit shows no cross-reactivity with any of the cytokines tested: Human Angiogenin, BDNF, BLC, ENA-78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
- Sensibilité
- 2 pg/mL
- Attributs du produit
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- Strip plates and additional reagents allow for use in multiple experiments
- Quantitative protein detection
- Establishes normal range
- The best products for confirmation of antibody array data
- Ingrédients
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- Pre-Coated 96-well Strip Microplate
- Wash Buffer
- Stop Solution
- Assay Diluent(s)
- Lyophilized Standard
- Biotinylated Detection Antibody
- Streptavidin-Conjugated HRP
- TMB One-Step Substrate
- Matériel non inclus
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- Distilled or deionized water
- Precision pipettes to deliver 2 μL to 1 μL volumes
- Adjustable 1-25 μL pipettes for reagent preparation
- 100 μL and 1 liter graduated cylinders
- Tubes to prepare standard and sample dilutions
- Absorbent paper
- Microplate reader capable of measuring absorbance at 450nm
- Log-log graph paper or computer and software for ELISA data analysis
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- Indications d'application
- Recommended Dilution for serum and plasma samples2 - 5 fold
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed in the manual.
- Add 100 μL of standard or sample to each well.
- Incubate 2.5 h at RT or O/N at 4 °C.
- Add 100 μL of prepared biotin antibody to each well.
- Incubate 1 h at RT.
- Add 100 μL of prepared Streptavidin solution to each well.
- Incubate 45 min at RT.
- Add 100 μL of TMB One-Step Substrate Reagent to each well.
- Incubate 30 min at RT.
- Add 50 μL of Stop Solution to each well.
- Read at 450 nm immediately.
- Restrictions
- For Research Use only
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- Stock
- -20 °C
- Stockage commentaire
- The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
- Date de péremption
- 6 months
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- Antigène Voir toutes KIT Ligand (KITLG) Kits ELISA
- KIT Ligand (KITLG)
- Autre désignation
- SCF (KITLG Produits)
- Synonymes
- Xkl-1 Kit ELISA, Xsl Kit ELISA, Xsl-1 Kit ELISA, Xsl-2 Kit ELISA, steel Kit ELISA, KITLG Kit ELISA, SCF Kit ELISA, kitl Kit ELISA, kl-1 Kit ELISA, mgf Kit ELISA, scf Kit ELISA, FPH2 Kit ELISA, KL-1 Kit ELISA, Kitl Kit ELISA, MGF Kit ELISA, SF Kit ELISA, SHEP7 Kit ELISA, Clo Kit ELISA, Con Kit ELISA, Gb Kit ELISA, Kitlg Kit ELISA, Mgf Kit ELISA, SLF Kit ELISA, Sl Kit ELISA, blz Kit ELISA, contrasted Kit ELISA, 710-712 Kit ELISA, CSF Kit ELISA, KITL Kit ELISA, KIT ligand L homeolog Kit ELISA, KIT ligand Kit ELISA, kit ligand Kit ELISA, kitlg.L Kit ELISA, KITLG Kit ELISA, kitlg Kit ELISA, Kitl Kit ELISA, Kitlg Kit ELISA
- Sujet
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Gene Names: KITLG MGF SCF
Protein names: Kit ligand (Mast cell growth factor) (MGF) (Stem cell factor) (SCF) (c-Kit ligand) [Cleaved into: Soluble KIT ligand (sKITLG)]
- ID gène
- 4254
- UniProt
- P21583
- Pathways
- Signalisation RTK, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
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