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IL10RB Kit ELISA
IL10RB
Reactivité: Humain
Colorimetric
Sandwich ELISA
5-3000 pg/mL
Cell Culture Supernatant, Plasma, Serum
Détail du produit IL10RB Kit ELISA
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Antigène
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IL10RB
(Interleukin 10 Receptor, beta (IL10RB))
Reactivité
Toutes les réactivités sur IL10RB Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
5-3000 pg/mL
Seuil minimal de détection
5 pg/mL
Application
ELISA
Fonction
Human IL-10 R beta ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, ENA-78, FGF- 4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, G-CSF, GM-CSF, IFN-gamma, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, SCF, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensibilité
5 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes IL10RB Kits ELISA
IL10RB
(Interleukin 10 Receptor, beta (IL10RB))
Autre désignation
IL-10 R beta (IL10RB Produits )
Synonymes
IL10R2 Kit ELISA, IL10RB Kit ELISA, il10r2 Kit ELISA, CDW210B Kit ELISA, CRF2-4 Kit ELISA, CRFB4 Kit ELISA, D21S58 Kit ELISA, D21S66 Kit ELISA, IL-10R2 Kit ELISA, 6620401D04Rik Kit ELISA, AI528744 Kit ELISA, Crfb4 Kit ELISA, D16H21S58 Kit ELISA, D21S58h Kit ELISA, Il10r2 Kit ELISA, RGD1560373 Kit ELISA, interleukin 10 receptor subunit beta Kit ELISA, interleukin 10 receptor subunit beta S homeolog Kit ELISA, interleukin-10 receptor subunit beta Kit ELISA, interleukin 10 receptor, beta Kit ELISA, IL10RB Kit ELISA, il10rb.S Kit ELISA, LOC473966 Kit ELISA, LOC478404 Kit ELISA, il10rb Kit ELISA, Il10rb Kit ELISA
Sujet
Gene Names: IL10RB CRFB4 D21S58 D21S66 Protein names: Interleukin-10 receptor subunit beta (IL-10 receptor subunit beta) (IL-10R subunit beta) (IL-10RB) (Cytokine receptor class-II member 4) (Cytokine receptor family 2 member 4) (CRF2-4) (Interleukin-10 receptor subunit 2) (IL-10R subunit 2) (IL-10R2) (CD antigen CDw210b)
ID gène
3588
UniProt
Q08334
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