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TNFRSF19 Kit ELISA
TNFRSF19
Reactivité: Humain
Colorimetric
Sandwich ELISA
50 pg/mL-400 ng/mL
Cell Culture Supernatant, Plasma, Serum
Détail du produit TNFRSF19 Kit ELISA
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Antigène
Voir toutes TNFRSF19 Kits ELISA
TNFRSF19
(Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))
Reactivité
Toutes les réactivités sur TNFRSF19 Kits ELISA
Humain
Méthode de détection
Colorimetric
Type de méthode
Sandwich ELISA
Gamme de detection
50 pg/mL-400 ng/mL
Seuil minimal de détection
50 pg/mL
Application
ELISA
Fonction
Human TROY (TNFRSF19) ELISA Kit for cell culture supernatants, plasma, and serum samples.
Type d'échantillon
Plasma, Cell Culture Supernatant, Serum
Analytical Method
Quantitative
Specificité
This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF, BLC, CNTF, ENA- 78, FGF-4, IL-1 alpha, IL-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, I-309, IP-10, FGF-4, FGF-6, FGF- 7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGFBP-3, IGFBP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1 alpha, MIP-1 beta, MIP-1 delta, PARC, PDGF, RANTES, SCF, SDF-1 alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF.
Sensibilité
50 pg/mL
Attributs du produit
Strip plates and additional reagents allow for use in multiple experiments Quantitative protein detection Establishes normal range The best products for confirmation of antibody array data
Ingrédients
Pre-Coated 96-well Strip Microplate Wash Buffer Stop Solution Assay Diluent(s) Lyophilized Standard Biotinylated Detection Antibody Streptavidin-Conjugated HRP TMB One-Step Substrate
Matériel non inclus
Distilled or deionized water Precision pipettes to deliver 2 μL to 1 μL volumes Adjustable 1-25 μL pipettes for reagent preparation 100 μL and 1 liter graduated cylinders Tubes to prepare standard and sample dilutions Absorbent paper Microplate reader capable of measuring absorbance at 450nm Log-log graph paper or computer and software for ELISA data analysis
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Information d'application
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Indications d'application
Recommended Dilution for serum and plasma samples2 - 10 fold
Volume d'échantillon
100 μL
Plaque
Pre-coated
Protocole
Prepare all reagents, samples and standards as instructed in the manual. Add 100 μL of standard or sample to each well. Incubate 2.5 h at RT or O/N at 4 °C. Add 100 μL of prepared biotin antibody to each well. Incubate 1 h at RT. Add 100 μL of prepared Streptavidin solution to each well. Incubate 45 min at RT. Add 100 μL of TMB One-Step Substrate Reagent to each well. Incubate 30 min at RT. Add 50 μL of Stop Solution to each well. Read at 450 nm immediately.
Restrictions
For Research Use only
Stockage
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Stock
-20 °C
Stockage commentaire
The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
Date de péremption
6 months
Détail du antigène
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Antigène
Voir toutes TNFRSF19 Kits ELISA
TNFRSF19
(Tumor Necrosis Factor Receptor Superfamily, Member 19 (TNFRSF19))
Autre désignation
TROY (TNFRSF19 Produits )
Synonymes
TNFRSF19 Kit ELISA, DKFZp468A0320 Kit ELISA, TAJ Kit ELISA, TAJ-alpha Kit ELISA, TRADE Kit ELISA, TROY Kit ELISA, AL023044 Kit ELISA, AW123854 Kit ELISA, TAJ-ALPHA Kit ELISA, Troy Kit ELISA, RGD1564996 Kit ELISA, TNF receptor superfamily member 19 Kit ELISA, tumor necrosis factor receptor superfamily, member 19 Kit ELISA, TNFRSF19 Kit ELISA, Tnfrsf19 Kit ELISA
Sujet
Gene Names: TNFRSF19 TAJ TROY UNQ1888/PRO4333 Protein names: Tumor necrosis factor receptor superfamily member 19 (TRADE) (Toxicity and JNK inducer)
ID gène
55504
UniProt
Q9NS68
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