HSP27 Kit ELISA

N° du produit ABIN625460

Détail du produit HSP27 Kit ELISA

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 0.15-50 ng/mL

Seuil minimal de détection: 0.15 ng/mL

Application: ELISA

Fonction: Human HSP27 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Plasma, Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Specificité: This ELISA antibody pair detect human HSP27. Other species not determined.

Sensibilité: 150 pg/mL

Attributs du produit:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Ingrédients:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Matériel non inclus:

• Distilled or deionized water
• Precision pipettes to deliver 2 μL to 1 μL volumes
• Adjustable 1-25 μL pipettes for reagent preparation
• 100 μL and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Information d'application

Indications d'application: Recommended Dilution for serum and plasma samples3 - 30 fold

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 μL of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4 °C.
4. Add 100 μL of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 μL of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 μL of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Stockage

Stock: -20 °C

Stockage commentaire: The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.

Date de péremption: 6 months

Détail du antigène

Antigène: HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Autre désignation: HSP27 (HSPB1 Produits)

Synonymes: CMT2F Kit ELISA, HMN2B Kit ELISA, HS.76067 Kit ELISA, HSP27 Kit ELISA, HSP28 Kit ELISA, Hsp25 Kit ELISA, SRP27 Kit ELISA, 27kDa Kit ELISA, Hsp27 Kit ELISA, cb153 Kit ELISA, cb660 Kit ELISA, hsp1 Kit ELISA, hsp25 Kit ELISA, hsp27 Kit ELISA, id:ibd2821 Kit ELISA, sb:cb660 Kit ELISA, zgc:103437 Kit ELISA, HSPB1 Kit ELISA, cmt2f Kit ELISA, hs.76067 Kit ELISA, hsp28 Kit ELISA, heat shock protein family B (small) member 1 Kit ELISA, heat shock protein 1 Kit ELISA, heat shock protein, alpha-crystallin-related, 1 Kit ELISA, heat shock protein family B (small) member 1 L homeolog Kit ELISA, HSPB1 Kit ELISA, Hspb1 Kit ELISA, hspb1 Kit ELISA, hspb1.L Kit ELISA

Sujet: Gene Names: HSPB1 HSP27 HSP28
Protein names: Heat shock protein beta-1 (HspB1) (28 kDa heat shock protein) (Estrogen-regulated 24 kDa protein) (Heat shock 27 kDa protein) (HSP 27) (Stress-responsive protein 27) (SRP27)

ID gène: 3315

UniProt: P04792

Pathways: Signalisation MAPK, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling