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BIRC7 Kit ELISA

BIRC7 Reactivité: Humain Colorimetric Sandwich ELISA Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625464
  • Antigène Voir toutes BIRC7 Kits ELISA
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    Reactivité
    • 6
    • 3
    • 2
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Application
    ELISA
    Fonction
    Human Livin ELISA Kit for Serum, Plasma, and Cell Culture Supernatants.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair recognizes Human Livin.
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
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  • Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Antigène Voir toutes BIRC7 Kits ELISA
    BIRC7 (Baculoviral IAP Repeat-Containing 7 (BIRC7))
    Autre désignation
    Livin (BIRC7 Produits)
    Synonymes
    KIAP Kit ELISA, LIVIN Kit ELISA, ML-IAP Kit ELISA, MLIAP Kit ELISA, RNF50 Kit ELISA, EIAP/XLX Kit ELISA, XLX Kit ELISA, kiap Kit ELISA, livin Kit ELISA, ml-iap Kit ELISA, mliap Kit ELISA, rnf50 Kit ELISA, xEIAP Kit ELISA, RGD1562883 Kit ELISA, zgc:165605 Kit ELISA, E130019N06 Kit ELISA, Livin Kit ELISA, birc7 Kit ELISA, bird7a Kit ELISA, xEIAP/XLX Kit ELISA, xlx Kit ELISA, EIAP/XLX-B Kit ELISA, birc7b Kit ELISA, baculoviral IAP repeat containing 7 Kit ELISA, baculoviral IAP repeat-containing 7 Kit ELISA, baculoviral IAP repeat-containing 7 (livin) Kit ELISA, baculoviral IAP repeat containing 7 S homeolog Kit ELISA, baculoviral IAP repeat containing 7 L homeolog Kit ELISA, BIRC7 Kit ELISA, birc7 Kit ELISA, Birc7 Kit ELISA, birc7.S Kit ELISA, birc7.L Kit ELISA
    ID gène
    79444
    UniProt
    Q96CA5
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