Tel:
+49 (0)241 95 163 153
Fax:
+49 (0)241 95 163 155
E-Mail:
orders@anticorps-enligne.fr

DIABLO Kit ELISA

DIABLO Reactivité: Humain Colorimetric Sandwich ELISA 0.1-30 ng/mL Cell Culture Supernatant, Plasma, Serum
N° du produit ABIN625468
  • Antigène Voir toutes DIABLO Kits ELISA
    DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
    Reactivité
    • 7
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    0.1-30 ng/mL
    Seuil minimal de détection
    0.1 ng/mL
    Application
    ELISA
    Fonction
    Human SMAC ELISA Kit for cell culture supernatants, plasma, and serum samples.
    Type d'échantillon
    Plasma, Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité
    This ELISA antibody pair detect human SMAC. Other species not determined yet.
    Sensibilité
    100 pg/mL
    Attributs du produit
    • Strip plates and additional reagents allow for use in multiple experiments
    • Quantitative protein detection
    • Establishes normal range
    • The best products for confirmation of antibody array data
    Ingrédients
    • Pre-Coated 96-well Strip Microplate
    • Wash Buffer
    • Stop Solution
    • Assay Diluent(s)
    • Lyophilized Standard
    • Biotinylated Detection Antibody
    • Streptavidin-Conjugated HRP
    • TMB One-Step Substrate
    Matériel non inclus
    • Distilled or deionized water
    • Precision pipettes to deliver 2 μL to 1 μL volumes
    • Adjustable 1-25 μL pipettes for reagent preparation
    • 100 μL and 1 liter graduated cylinders
    • Tubes to prepare standard and sample dilutions
    • Absorbent paper
    • Microplate reader capable of measuring absorbance at 450nm
    • Log-log graph paper or computer and software for ELISA data analysis
    Featured
    Discover our best selling DIABLO Kit ELISA
    Top Product
    Discover our top product DIABLO Kit ELISA
  • Indications d'application
    Recommended Dilution for serum and plasma samples2 fold
    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Protocole
    1. Prepare all reagents, samples and standards as instructed in the manual.
    2. Add 100 μL of standard or sample to each well.
    3. Incubate 2.5 h at RT or O/N at 4 °C.
    4. Add 100 μL of prepared biotin antibody to each well.
    5. Incubate 1 h at RT.
    6. Add 100 μL of prepared Streptavidin solution to each well.
    7. Incubate 45 min at RT.
    8. Add 100 μL of TMB One-Step Substrate Reagent to each well.
    9. Incubate 30 min at RT.
    10. Add 50 μL of Stop Solution to each well.
    11. Read at 450 nm immediately.
    Restrictions
    For Research Use only
  • Stock
    -20 °C
    Stockage commentaire
    The entire kit may be stored at -20°C for up to 1 year from the date of shipment. Avoid repeated freeze-thaw cycles. The kit may be stored at 4°C for up to 6 months. For extended storage, it is recommended to store at -80°C.
    Date de péremption
    6 months
  • Wiita, Hsu, Lu, Esensten, Wells: "Circulating proteolytic signatures of chemotherapy-induced cell death in humans discovered by N-terminal labeling." dans: Proceedings of the National Academy of Sciences of the United States of America, Vol. 111, Issue 21, pp. 7594-9, (2014) (PubMed).

  • Antigène Voir toutes DIABLO Kits ELISA
    DIABLO (Second Mitochondria-Derived Activator of Caspase (DIABLO))
    Autre désignation
    SMAC (DIABLO Produits)
    Synonymes
    smac Kit ELISA, smac3 Kit ELISA, diablo-s Kit ELISA, 0610041G12Rik Kit ELISA, 1700006L01Rik Kit ELISA, AU040403 Kit ELISA, Smac Kit ELISA, DFNA64 Kit ELISA, SMAC Kit ELISA, DBO Kit ELISA, XSmac Kit ELISA, diablo, IAP-binding mitochondrial protein Kit ELISA, diablo IAP-binding mitochondrial protein Kit ELISA, diablo, IAP-binding mitochondrial protein L homeolog Kit ELISA, diablo Kit ELISA, Diablo Kit ELISA, DIABLO Kit ELISA, diablo.L Kit ELISA
    Sujet
    Gene Names: DIABLO SMAC
    Protein names: Diablo homolog, mitochondrial (Direct IAP-binding protein with low pI) (Second mitochondria-derived activator of caspase) (Smac)
    ID gène
    56616
    UniProt
    Q9NR28
    Pathways
    Apoptose, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity
Vous êtes ici:
Support technique