ACPP Kit IQ-ELISA
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- Antigène Voir toutes ACPP IQ-ELISA Kits
- ACPP (Acid Phosphatase, Prostate (ACPP))
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Reactivité
- Humain
- Méthode de détection
- qPCR
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Human Immunoquantitative (PCR-Based) ACPP ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- Attributs du produit
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- Ingrédients
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- ACPP Microplate (Item A): 96 well PCR plate coated with anti-Human ACPP
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Human ACPP
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for ACPP (Item F): 2 vials of a 4x concentrated solution of anti-Human ACPP affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- Matériel non inclus
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
- Featured
- Discover our best selling ACPP Kit ELISA
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- Indications d'application
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- Commentaires
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- Volume d'échantillon
- 25 µL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- Date de péremption
- 6 months
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- Antigène Voir toutes ACPP IQ-ELISA Kits
- ACPP (Acid Phosphatase, Prostate (ACPP))
- Autre désignation
- ACPP (ACPP Produits)
- Synonymes
- 5'-NT Kit IQ-ELISA, ACP-3 Kit IQ-ELISA, ACP3 Kit IQ-ELISA, PAP Kit IQ-ELISA, AMAP2 Kit IQ-ELISA, CENTB3 Kit IQ-ELISA, DDEF2 Kit IQ-ELISA, PAG3 Kit IQ-ELISA, Pap-alpha Kit IQ-ELISA, SHAG1 Kit IQ-ELISA, A030005E02Rik Kit IQ-ELISA, FRAP Kit IQ-ELISA, Lap Kit IQ-ELISA, Ppal Kit IQ-ELISA, Acpp11 Kit IQ-ELISA, RNACPP11 Kit IQ-ELISA, pap Kit IQ-ELISA, ACPP Kit IQ-ELISA, acp-3 Kit IQ-ELISA, acp3 Kit IQ-ELISA, acpt Kit IQ-ELISA, acid phosphatase, prostate Kit IQ-ELISA, ArfGAP with SH3 domain, ankyrin repeat and PH domain 2 Kit IQ-ELISA, prostatic acid phosphatase Kit IQ-ELISA, prostatic acid phosphatase, putative Kit IQ-ELISA, acid phosphatase, prostate S homeolog Kit IQ-ELISA, ACPP Kit IQ-ELISA, ASAP2 Kit IQ-ELISA, Acpp Kit IQ-ELISA, CpipJ_CPIJ004002 Kit IQ-ELISA, Smp_016640 Kit IQ-ELISA, acpp.S Kit IQ-ELISA
- ID gène
- 55
- UniProt
- P15309
- Pathways
- Synaptic Membrane, Ribonucleoside Biosynthetic Process
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