CD40 Ligand Kit IQ-ELISA
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- Antigène Voir toutes CD40 Ligand (CD40LG) IQ-ELISA Kits
- CD40 Ligand (CD40LG)
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Reactivité
- Souris, Rat
- Méthode de détection
- qPCR
- Type de méthode
- Sandwich ELISA
- Application
- ELISA
- Fonction
- Rat Immunoquantitative (PCR-Based) CD40 Ligand (TNFSF5 ELISA Kit for cell culture supernatants, plasma, and serum samples.
- Type d'échantillon
- Cell Culture Supernatant, Plasma, Serum
- Analytical Method
- Semi-Quantitative
- Sensibilité
- 0.045 ng/mL
- Attributs du produit
- IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.
- Ingrédients
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- CD40 Ligand (TNFSF5 Microplate (Item A): 96 well PCR plate coated with anti-Rat CD40 Ligand (TNFSF5
- Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
- Standards (Item C): 2 vials of recombinant Rat CD40 Ligand (TNFSF5
- Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
- Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
- Detection Affinity Reagent for CD40 Ligand (TNFSF5 (Item F): 2 vials of a 4x concentrated solution of anti-Rat CD40 Ligand (TNFSF5 affinity reagent
- IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
- Primer Solution (Item I): 1.7 ml vial
- PCR Master Mix (Item J): 1.2 ml vial
- PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
- Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer
- Matériel non inclus
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- Real-time PCR instrument, Bio-Rad recommended
- Precision pipettes to deliver 2 μL to 1 mL volumes
- Adjustable 1-25 mL pipettes for reagent preparation
- 100 mL and 1 liter graduated cylinders
- Absorbent paper
- Distilled or deionized water
- Log-log graph paper or computer and software for data analysis
- Tubes to prepare standard or sample dilutions
- Heating block or water bath capable of 80°C
- Featured
- Discover our best selling CD40LG Kit ELISA
- Top Product
- Discover our top product CD40LG Kit ELISA
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- Indications d'application
- The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.
- Commentaires
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The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.
- Volume d'échantillon
- 25 µL
- Plaque
- Pre-coated
- Protocole
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- Prepare all reagents, samples and standards as instructed
- Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4°C
- Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
- Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
- Add 15μL Primer solution 10μL of PCR master mix to each well
- Run real-time PCR
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C,-80 °C
- Stockage commentaire
- May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.
- Date de péremption
- 6 months
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- Antigène Voir toutes CD40 Ligand (CD40LG) IQ-ELISA Kits
- CD40 Ligand (CD40LG)
- Autre désignation
- CD40 Ligand (TNFSF5) (CD40LG Produits)
- Synonymes
- CD154 Kit IQ-ELISA, cd40l Kit IQ-ELISA, CD40L Kit IQ-ELISA, HIGM1 Kit IQ-ELISA, IGM Kit IQ-ELISA, IMD3 Kit IQ-ELISA, T-BAM Kit IQ-ELISA, TNFSF5 Kit IQ-ELISA, TRAP Kit IQ-ELISA, gp39 Kit IQ-ELISA, hCD40L Kit IQ-ELISA, CD40-L Kit IQ-ELISA, Cd40l Kit IQ-ELISA, Ly-62 Kit IQ-ELISA, Ly62 Kit IQ-ELISA, Tnfsf5 Kit IQ-ELISA, CD40 ligand Kit IQ-ELISA, TNF superfamily member 5 Kit IQ-ELISA, CD40LG Kit IQ-ELISA, cd40l Kit IQ-ELISA, Cd40lg Kit IQ-ELISA
- ID gène
- 84349
- UniProt
- Q9Z2V2
- Pathways
- Signalisation NF-kappaB, Production of Molecular Mediator of Immune Response, Cancer Immune Checkpoints
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