Thymidine Phosphorylase Kit IQ-ELISA

N° du produit ABIN6384482

Détail du produit Thymidine Phosphorylase Kit IQ-ELISA

Antigène: Thymidine Phosphorylase (TYMP)

Reactivité: Humain

Méthode de détection: qPCR

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Immunoquantitative (PCR-Based) PD-ECGF ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensibilité: 0.04 ng/mL

Attributs du produit: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Ingrédients:

• PD-ECGF Microplate (Item A): 96 well PCR plate coated with anti-Human PD-ECGF
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human PD-ECGF
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for PD-ECGF (Item F): 2 vials of a 4x concentrated solution of anti-Human PD-ECGF affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Commentaires:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Volume d'échantillon: 25 µL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: Thymidine Phosphorylase (TYMP)

Autre désignation: PD-ECGF (TYMP Produits)

Synonymes: ECGF Kit IQ-ELISA, ECGF1 Kit IQ-ELISA, MEDPS1 Kit IQ-ELISA, MNGIE Kit IQ-ELISA, MTDPS1 Kit IQ-ELISA, PDECGF Kit IQ-ELISA, TP Kit IQ-ELISA, hPD-ECGF Kit IQ-ELISA, 2900072D10Rik Kit IQ-ELISA, Ecgf1 Kit IQ-ELISA, PD-ECGF Kit IQ-ELISA, Pdgfec Kit IQ-ELISA, typh Kit IQ-ELISA, thymidine phosphorylase Kit IQ-ELISA, thymidine phosphorylase DeoA Kit IQ-ELISA, AMP phosphorylase Kit IQ-ELISA, Thymidine phosphorylase Kit IQ-ELISA, TYMP Kit IQ-ELISA, Tymp Kit IQ-ELISA, deoA Kit IQ-ELISA, Dd586_0614 Kit IQ-ELISA, Kvar_4413 Kit IQ-ELISA, NMAG_RS19330 Kit IQ-ELISA, Snov_1187 Kit IQ-ELISA, Fbal_2856 Kit IQ-ELISA, Ftrac_2071 Kit IQ-ELISA, Intca_1216 Kit IQ-ELISA, ARCVE_RS10415 Kit IQ-ELISA, Sphch_4174 Kit IQ-ELISA, Sinme_3461 Kit IQ-ELISA, METOK_RS06355 Kit IQ-ELISA, Mesop_1831 Kit IQ-ELISA, typh Kit IQ-ELISA

ID gène: 1890

UniProt: P19971

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2