GREM1 Kit IQ-ELISA

N° du produit ABIN6384552

Détail du produit GREM1 Kit IQ-ELISA

Antigène: GREM1 (Gremlin 1 (GREM1))

Reactivité: Souris

Méthode de détection: qPCR

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Mouse Immunoquantitative (PCR-Based) Gremlin-1 ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensibilité: 0.08 ng/mL

Attributs du produit: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Ingrédients:

• Gremlin-1 Microplate (Item A): 96 well PCR plate coated with anti-Mouse Gremlin-1
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Mouse Gremlin-1
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Gremlin-1 (Item F): 2 vials of a 4x concentrated solution of anti-Mouse Gremlin-1 affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Commentaires:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Volume d'échantillon: 25 µL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: GREM1 (Gremlin 1 (GREM1))

Autre désignation: Gremlin-1 (GREM1 Produits)

Synonymes: grem1 Kit IQ-ELISA, MGC136702 Kit IQ-ELISA, zgc:136702 Kit IQ-ELISA, GREM1 Kit IQ-ELISA, drm Kit IQ-ELISA, pig2 Kit IQ-ELISA, dand2 Kit IQ-ELISA, ihg-2 Kit IQ-ELISA, gremlin Kit IQ-ELISA, Cktsf1b1 Kit IQ-ELISA, Drm Kit IQ-ELISA, Grem Kit IQ-ELISA, ld Kit IQ-ELISA, gremlin-1 Kit IQ-ELISA, CKTSF1B1 Kit IQ-ELISA, DAND2 Kit IQ-ELISA, DRM Kit IQ-ELISA, GREMLIN Kit IQ-ELISA, IHG-2 Kit IQ-ELISA, cktsf1b1 Kit IQ-ELISA, gremlin 1a, DAN family BMP antagonist Kit IQ-ELISA, gremlin 1, DAN family BMP antagonist Kit IQ-ELISA, gremlin 1 Kit IQ-ELISA, gremlin 1, DAN family BMP antagonist L homeolog Kit IQ-ELISA, grem1a Kit IQ-ELISA, GREM1 Kit IQ-ELISA, LOC662541 Kit IQ-ELISA, grem1 Kit IQ-ELISA, Grem1 Kit IQ-ELISA, grem1.L Kit IQ-ELISA

ID gène: 23892

UniProt: O70326

Pathways: Regulation of Muscle Cell Differentiation, Tube Formation, Maintenance of Protein Location