RPS6KB1 Kit IQ-ELISA

N° du produit ABIN6384888

Détail du produit RPS6KB1 Kit IQ-ELISA

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Reactivité: Humain

Méthode de détection: qPCR

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Immunoquantitative (PCR-Based) p70 S6 Kinase ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Attributs du produit: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Ingrédients:

• p70 S6 Kinase Microplate (Item A): 96 well PCR plate coated with anti-Human p70 S6 Kinase
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human p70 S6 Kinase
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for p70 S6 Kinase (Item F): 2 vials of a 4x concentrated solution of anti-Human p70 S6 Kinase affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Commentaires:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Volume d'échantillon: 25 µL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: RPS6KB1 (Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 1 (RPS6KB1))

Autre désignation: p70 S6 Kinase (RPS6KB1 Produits)

Synonymes: PS6K Kit IQ-ELISA, S6K Kit IQ-ELISA, S6K-beta-1 Kit IQ-ELISA, S6K1 Kit IQ-ELISA, STK14A Kit IQ-ELISA, p70 S6KA Kit IQ-ELISA, p70(S6K)-alpha Kit IQ-ELISA, p70-S6K Kit IQ-ELISA, p70-alpha Kit IQ-ELISA, 10539 Kit IQ-ELISA, 7084 Kit IQ-ELISA, CG10539 Kit IQ-ELISA, DS6K Kit IQ-ELISA, Dmel\\CG10539 Kit IQ-ELISA, Dp70S6k Kit IQ-ELISA, Dp70[s6k] Kit IQ-ELISA, Dp70s6k Kit IQ-ELISA, S6k Kit IQ-ELISA, dS6K Kit IQ-ELISA, dS6k Kit IQ-ELISA, dp70[S6k] Kit IQ-ELISA, dp70s6k Kit IQ-ELISA, dps6k Kit IQ-ELISA, ds6k Kit IQ-ELISA, fs(3)07084 Kit IQ-ELISA, l(3)07084 Kit IQ-ELISA, p70 S6K Kit IQ-ELISA, p70/S6K Kit IQ-ELISA, p70S6K Kit IQ-ELISA, p70[S6 kinase] Kit IQ-ELISA, p70[S6K] Kit IQ-ELISA, p70[S6k] Kit IQ-ELISA, p70[S6kinase] Kit IQ-ELISA, p70s6K Kit IQ-ELISA, s6k Kit IQ-ELISA, s6k11 Kit IQ-ELISA, 2610318I15Rik Kit IQ-ELISA, 4732464A07Rik Kit IQ-ELISA, 70kDa Kit IQ-ELISA, AA959758 Kit IQ-ELISA, AI256796 Kit IQ-ELISA, AI314060 Kit IQ-ELISA, p70/85s6k Kit IQ-ELISA, p70s6k Kit IQ-ELISA, p70s6k-A Kit IQ-ELISA, p70-s6k Kit IQ-ELISA, ps6k Kit IQ-ELISA, rps6kb1 Kit IQ-ELISA, rps6kb1-A Kit IQ-ELISA, s6K1 Kit IQ-ELISA, stk14a Kit IQ-ELISA, fc51h01 Kit IQ-ELISA, wu:fc51h01 Kit IQ-ELISA, zgc:55713 Kit IQ-ELISA, ribosomal protein S6 kinase B1 Kit IQ-ELISA, Ribosomal protein S6 kinase Kit IQ-ELISA, ribosomal protein S6 kinase, polypeptide 1 Kit IQ-ELISA, ribosomal protein S6 kinase B1 L homeolog Kit IQ-ELISA, ribosomal protein S6 kinase B1 S homeolog Kit IQ-ELISA, ribosomal protein S6 kinase b, polypeptide 1b Kit IQ-ELISA, RPS6KB1 Kit IQ-ELISA, S6k Kit IQ-ELISA, Rps6kb1 Kit IQ-ELISA, rps6kb1.L Kit IQ-ELISA, rps6kb1.S Kit IQ-ELISA, rps6kb1b Kit IQ-ELISA

ID gène: 6198

UniProt: P23443

Pathways: Signalisation PI3K-Akt, Signalisation RTK, AMPK Signaling, Regulation of Cell Size, Skeletal Muscle Fiber Development, Feeding Behaviour, G-protein mediated Events, Smooth Muscle Cell Migration, Interaction of EGFR with phospholipase C-gamma, L'effet Warburg