c-MYC Kit IQ-ELISA

N° du produit ABIN6385413

Détail du produit c-MYC Kit IQ-ELISA

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Reactivité: Humain

Méthode de détection: qPCR

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Immunoquantitative (PCR-Based) c-Myc, MYC ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensibilité: 0.062 ng/mL

Attributs du produit: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Ingrédients:

• c-Myc///MYC Microplate (Item A): 96 well PCR plate coated with anti-Human c-Myc///MYC
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human c-Myc///MYC
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for c-Myc///MYC (Item F): 2 vials of a 4x concentrated solution of anti-Human c-Myc///MYC affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Commentaires:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Volume d'échantillon: 25 µL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: c-MYC (MYC) (Myc Proto-Oncogene protein (MYC))

Autre désignation: c-Myc///MYC (MYC Produits)

Synonymes: MRTL Kit IQ-ELISA, MYCC Kit IQ-ELISA, bHLHe39 Kit IQ-ELISA, c-Myc Kit IQ-ELISA, CMYC Kit IQ-ELISA, c-myc Kit IQ-ELISA, C-MYC Kit IQ-ELISA, LOC100136746 Kit IQ-ELISA, ARC Kit IQ-ELISA, B430311C09Rik Kit IQ-ELISA, MYC Kit IQ-ELISA, NOP Kit IQ-ELISA, Nop30 Kit IQ-ELISA, RNCMYC Kit IQ-ELISA, mMyc Kit IQ-ELISA, AU016757 Kit IQ-ELISA, Myc2 Kit IQ-ELISA, Niard Kit IQ-ELISA, Nird Kit IQ-ELISA, cmyc Kit IQ-ELISA, zc-myc Kit IQ-ELISA, c-myc II Kit IQ-ELISA, myc-B Kit IQ-ELISA, myc2 Kit IQ-ELISA, myc1 Kit IQ-ELISA, CMyc Kit IQ-ELISA, zgc:55680 Kit IQ-ELISA, CG10798 Kit IQ-ELISA, D-Myc Kit IQ-ELISA, DM Kit IQ-ELISA, DMYc Kit IQ-ELISA, Dm Kit IQ-ELISA, Dmel\CG10798 Kit IQ-ELISA, Dmyc Kit IQ-ELISA, EG:BACN5I9.1 Kit IQ-ELISA, anon-WO03040301.171 Kit IQ-ELISA, bHLHe57 Kit IQ-ELISA, c-MYC Kit IQ-ELISA, d-myc Kit IQ-ELISA, dMYC Kit IQ-ELISA, dMyc Kit IQ-ELISA, dMyc1 Kit IQ-ELISA, da Kit IQ-ELISA, dm Kit IQ-ELISA, dm/dMyc Kit IQ-ELISA, dm/myc Kit IQ-ELISA, dmyc Kit IQ-ELISA, dmyc1 Kit IQ-ELISA, l(1)G0139 Kit IQ-ELISA, l(1)G0354 Kit IQ-ELISA, l(1)G0359 Kit IQ-ELISA, myc Kit IQ-ELISA, MYC proto-oncogene, bHLH transcription factor Kit IQ-ELISA, v-myc myelocytomatosis viral oncogene homolog Kit IQ-ELISA, nucleolar protein 3 (apoptosis repressor with CARD domain) Kit IQ-ELISA, myelocytomatosis oncogene Kit IQ-ELISA, MYC proto-oncogene, bHLH transcription factor a Kit IQ-ELISA, MYC proto-oncogene, bHLH transcription factor L homeolog Kit IQ-ELISA, MYC proto-oncogene, bHLH transcription factor S homeolog Kit IQ-ELISA, v-myc myelocytomatosis viral oncogene homolog (avian) Kit IQ-ELISA, MYC proto-oncogene, bHLH transcription factor b Kit IQ-ELISA, CG10798 gene product from transcript CG10798-RB Kit IQ-ELISA, MYC Kit IQ-ELISA, LOC100136746 Kit IQ-ELISA, Nol3 Kit IQ-ELISA, Myc Kit IQ-ELISA, myca Kit IQ-ELISA, myc.L Kit IQ-ELISA, myc.S Kit IQ-ELISA, mycb Kit IQ-ELISA

ID gène: 4609

UniProt: P01106

Pathways: Signalisation p53, Cycle Cellulaire, Sensory Perception of Sound, Transition Metal Ion Homeostasis, Mitotic G1-G1/S Phases, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, L'effet Warburg