PPIA Kit IQ-ELISA

N° du produit ABIN6385528

Détail du produit PPIA Kit IQ-ELISA

Antigène: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Reactivité: Humain

Méthode de détection: qPCR

Type de méthode: Sandwich ELISA

Application: ELISA

Fonction: Human Immunoquantitative (PCR-Based) Cyclophilin A ELISA Kit for cell culture supernatants, plasma, and serum samples.

Type d'échantillon: Cell Culture Supernatant, Plasma, Serum

Analytical Method: Semi-Quantitative

Sensibilité: 0.205 ng/mL

Attributs du produit: IQ-ELISAs employ specific capture antibodies coated on a 96-well PCR plate. Standards and samples are pipetted into the wells, the target protein in the standards and samples binds to the immobilized antibody. The wells are washed and the detection affinity reagent is added to the wells where it binds to any captured antigen. The wells are washed, and primers and PCR master mix are added to each well. The plate is placed into a real time PCR instrument for cycling and measurement of DNA amplification. The cycle number where amplification is detected is proportional to the amount of affinity detection reagent that bound to captured antigen in each well.

Ingrédients:

• Cyclophilin A Microplate (Item A): 96 well PCR plate coated with anti-Human Cyclophilin A
• Wash Buffer I Concentrate (20x) (Item B): 25 ml of 20x concentrated solution
• Standards (Item C): 2 vials of recombinant Human Cyclophilin A
• Assay Diluent A (Item D): 30 ml diluent buffer, 0.09% sodium azide as preservative. For Standard/Sample (serum/plasma) diluent
• Assay Diluent B (Item E): 15 ml of 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent
• Detection Affinity Reagent for Cyclophilin A (Item F): 2 vials of a 4x concentrated solution of anti-Human Cyclophilin A affinity reagent
• IQELISA Detection Reagent (Item G): 1.4ml of a 10x concentrated stock
• Primer Solution (Item I): 1.7 ml vial
• PCR Master Mix (Item J): 1.2 ml vial
• PCR Preparation buffer (Item K): 1ml vial of 10x concentrated buffer
• Final Wash Buffer (Item L): 10ml vial of 10x concentrated buffer

Matériel non inclus:

• Real-time PCR instrument, Bio-Rad recommended
• Precision pipettes to deliver 2 μL to 1 mL volumes
• Adjustable 1-25 mL pipettes for reagent preparation
• 100 mL and 1 liter graduated cylinders
• Absorbent paper
• Distilled or deionized water
• Log-log graph paper or computer and software for data analysis
• Tubes to prepare standard or sample dilutions
• Heating block or water bath capable of 80^°C

Information d'application

Indications d'application: The Immuno-Quantitative ELISA (IQELISA) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. This results in an assay that is simultaneously familiar and cutting edge and enables the use of only 1/10th the sample volume while also providing 10x more sensitivity than a traditional ELISA.

Commentaires:

The Immuno-Quantitative ELISA (IQELISA™) kits are an innovative assay platform that combines the specificity and ease of use of an ELISA with the sensitivity of real-time PCR. Also called immuno-PCR, this detection platform results in an assay that is simultaneously familiar and cutting edge. Compared to traditional ELISA, IQELISA™ enables the use of only 1/10th the sample volume while also providing 10x more sensitivity.

Volume d'échantillon: 25 µL

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards as instructed
2. Add 25 μL standard or sample to each well. Incubate for 2.5 hours at room temperature or overnight at 4^°C
3. Add 25 μL detection affinity reagent to each well. Incubate 1 hour at room temperature
4. Add 25μL of IQELISA Detection Reagent to each well. Incubate 1 hour
5. Add 15μL Primer solution 10μL of PCR master mix to each well
6. Run real-time PCR

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C,-80 °C

Stockage commentaire: May be stored for up to 6 months at 2° to 8°C from the date of shipment. Standard (recombinant protein) should be stored at -20°C or -80°C (recommended at -80°C) after reconstitution. Opened PCR plate or reagents may be stored for up to 1 month at 2° to 8°C. Note: the kit can be used within one year if the whole kit is stored at -20°C. Avoid repeated freeze-thaw cycles.

Date de péremption: 6 months

Détail du antigène

Antigène: PPIA (Peptidylprolyl Isomerase A (Cyclophilin A) (PPIA))

Autre désignation: Cyclophilin A (PPIA Produits)

Synonymes: Cyp1 Kit IQ-ELISA, PPIA-2 Kit IQ-ELISA, fa93g09 Kit IQ-ELISA, ppia Kit IQ-ELISA, wu:fa93g09 Kit IQ-ELISA, wu:fb05e11 Kit IQ-ELISA, wu:fb13h02 Kit IQ-ELISA, wu:fb15a05 Kit IQ-ELISA, CYPA Kit IQ-ELISA, CypA Kit IQ-ELISA, Cypa Kit IQ-ELISA, CYPH Kit IQ-ELISA, CYCA Kit IQ-ELISA, CyP-A Kit IQ-ELISA, MGC75715 Kit IQ-ELISA, 2700098C05 Kit IQ-ELISA, Cphn Kit IQ-ELISA, CyP-18 Kit IQ-ELISA, MT-ND1 Kit IQ-ELISA, MTND1 Kit IQ-ELISA, NADH1 Kit IQ-ELISA, ND1 Kit IQ-ELISA, cypA Kit IQ-ELISA, PPIase A Kit IQ-ELISA, ppial Kit IQ-ELISA, wu:fj18g05 Kit IQ-ELISA, zgc:73102 Kit IQ-ELISA, zgc:86688 Kit IQ-ELISA, peptidylprolyl isomerase A Kit IQ-ELISA, peptidylprolyl isomerase Aa (cyclophilin A) Kit IQ-ELISA, peptidylprolyl isomerase A (cyclophilin A) L homeolog Kit IQ-ELISA, cyclophilin A Kit IQ-ELISA, cyclophilin a Kit IQ-ELISA, peptidylprolyl isomerase A (cyclophilin A) Kit IQ-ELISA, peptidylprolyl isomerase Ab (cyclophilin A) Kit IQ-ELISA, PPIA Kit IQ-ELISA, ppiaa Kit IQ-ELISA, ppia.L Kit IQ-ELISA, Cypa Kit IQ-ELISA, CNB01230 Kit IQ-ELISA, CNB01290 Kit IQ-ELISA, Tc00.1047053506925.300 Kit IQ-ELISA, Tb11.03.0250 Kit IQ-ELISA, CYPA Kit IQ-ELISA, Ppia Kit IQ-ELISA, ppia Kit IQ-ELISA, ppiab Kit IQ-ELISA

ID gène: 5478

UniProt: P62937