Luteinizing Hormone Kit ELISA

N° du produit ABIN6574077

Détail du produit Luteinizing Hormone Kit ELISA

Antigène: Luteinizing Hormone (LH)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Competition ELISA

Gamme de detection: 370.4 pg/mL - 30000 pg/mL

Seuil minimal de détection: 370.4 pg/mL

Application: ELISA

Fonction: The kit is a competitive inhibition enzyme immunoassay technique for the in vitro quantitative measurement of luteinizing hormone in mouse serum, plasma.

We offer validation data (WB) for the kit components. So you can be sure to order a reliable ELISA kit product composed of high quality reagents.

Type d'échantillon: Plasma, Serum

Analytical Method: Quantitative

Specificité: This assay has high sensitivity and excellent specificity for detection of Luteinizing Hormone (LH)

Réactivité croisée (Details): No significant cross-reactivity or interference between Luteinizing Hormone (LH) and analogues was observed.

Sensibilité: 145.2 pg/mL

Ingrédients:

• Pre-coated, ready to use 96-well strip plate, flat buttom
• Plate sealer for 96 wells
• Reference Standard
• Standard Diluent
• Detection Reagent A
• Detection Reagent B
• Assay Diluent A
• Assay Diluent B
• Reagent Diluent (if Detection Reagent is lyophilized)
• TMB Substrate
• Stop Solution
• Wash Buffer (30 x concentrate)
• Instruction manual

Information d'application

Commentaires:

Information on standard material:
The standard might be recombinant protein or natural protein, that will depend on the specific kit. Moreover, the expression system is E.coli or yeast or mammal cell. There is 0.05% proclin 300 in the standard as preservative.

Information on reagents:
The stop solution used in the kit is sulfuric acid with concentration of 1 mol/L. And the wash solution is TBS. The standard diluent contains 0.02 % sodium azide, assay diluent A and assay diluent B contain 0.01% sodium azide. Some kits can contain is BSA in them.

Information on antibodies:
The provided antibodies and their host vary in different kits.

Volume d'échantillon: 50 μL

Durée du test: 2 h

Plaque: Pre-coated

Protocole:

1. Prepare all reagents, samples and standards,
2. Add 50μL standard or sample to each well.
   Then add 50μL prepared Detection Reagent A immediately.
   Shake and mix. Incubate 1 hour at 37 °C,
3. Aspirate and wash 3 times,
4. Add 100μL prepared Detection Reagent B. Incubate 30 minutes at 37 °C,
5. Aspirate and wash 5 times,
6. Add 90μL Substrate Solution. Incubate 10-20 minutes at 37 °C,
7. Add 50μL Stop Solution. Read at 450 nm immediately.

Préparation des réactifs:

1. Bring all kit components and samples to room temperature (18-25 °C) before use. If the kit will not be used up in one time, please only take out strips and reagents for present experiment, and leave the remaining strips and reagents in required condition.
2. Standard - Reconstitute the Standard with 0.5 mL of Standard Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). The concentration of the standard in the stock solution is 30,000pg/mL. Please prepare 5 tubes containing 0.6 mL Standard Diluent and produce a triple dilution series according to the picture shown below. Mix each tube thoroughly before the next transfer. Set up 5 points of diluted standard such as 30,000pg/mL, 10,000pg/mL, 3,333.3pg/mL, 1,111.1pg/mL, 370.4pg/mL, and the last EP tubes with Standard Diluent is the blank as 0pg/mL.
3. Detection Reagent A - Reconstitute the Detection Reagent A with 150μL of Reagent Diluent, kept for 10 minutes at room temperature, shake gently(not to foam). Dilute to the working concentration with Assay Diluent A (1:100).
4. Detection Reagent B - Briefly spin or centrifuge the stock Detection B before use. Dilute to the working concentration with Assay Diluent B (1:50).
5. Wash Solution - Dilute 20 mL of Wash Solution concentrate (30x) with 580 mL of deionized or distilled water to prepare 600 mL of Wash Solution (1x).
6. TMB substrate - Aspirate the needed dosage of the solution with sterilized tips and do not dump the residual solution into the vial again.

Note:

1. Making serial dilution in the wells directly is not permitted.
2. Prepare standard within 15 minutes before assay. Please do not dissolve the reagents at 37 °C directly.
3. Detection Reagent A and B are sticky solutions, therefore, slowly pipette them to reduce the volume errors.
4. Please carefully reconstitute Standards or working Detection Reagent A and B according to the instruction, and avoid foaming and mix gently until the crystals are completely dissolved. To minimize imprecision caused by pipetting, use small volumes and ensure that pipettors are calibrated. It is recommended to suck more than 10μL for one pipetting.
5. The reconstituted Standards, Detection Reagent A and Detection Reagent B can be used only once.
6. If crystals have formed in the Wash Solution concentrate (30x), warm to room temperature and mix gently until the crystals are completely dissolved.
7. Contaminated water or container for reagent preparation will influence the detection result.

Préparation de l'échantillon:

• It is recommended to use fresh samples without long storage, otherwise protein degradation and denaturation may occur in these samples, leading to false results. Samples should therefore be stored for a short period at 2 - 8 °C or aliquoted at -20 °C (≤1 month) or -80 °C (≤ 3 months). Repeated freeze-thaw cycles should be avoided. Prior to assay, the frozen samples should be slowly thawed and centrifuged to remove precipitates.
• If the sample type is not specified in the instructions, a preliminary test is necessary to determine compatibility with the kit.
• If a lysis buffer is used to prepare tissue homogenates or cell culture supernatant, there is a possibility of causing a deviation due to the introduced chemical substance. The recommended dilution factor is for reference only.
• Please estimate the concentration of the samples before performing the test. If the values are not in the range of the standard curve, the optimal sample dilution for the particular experiment has to be determined.

Précision du teste: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level of target were tested 20 times on one plate, respectively.
Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level of target were tested on 3 different plates, 8 replicates in each plate.
CV(%) = SD/meanX100
Intra-Assay: CV < 10%
Inter-Assay: CV < 12%

Restrictions: For Research Use only

Stockage

Précaution d'utilisation: The Stop Solution suggested for use with this kit is an acid solution. Wear eye, hand, face, and clothing protection when using this material.

Stock: 4 °C/-20 °C

Stockage commentaire:

1. For unopened kit: All reagents should be stored according to the labels on the vials. The Standard, Detection Reagent A, Detection Reagent B, and 96-well Strip Plate should be stored at -20 °C upon receipt, while the other reagents should be stored at 4 °C.
2. For opened kits: the remaining reagents must be stored according to the above storage conditions. In addition, please return the unused wells to the foil pouch containing the desiccant and seal the foil pouch with the zipper.

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Date de péremption: 6 months

Références pour Luteinizing Hormone Kit ELISA (ABIN6574077)

Isola, Veiga, de Brito, Alvarado-Rincón, Garcia, Zanini, Hense, Vieira, Garratt, Gasperin, Schneider, Stout: "17α-estradiol does not adversely affect sperm parameters or fertility in male mice: implications for reproduction-longevity trade-offs." dans: GeroScience, (2022) (PubMed).

Li, Ma, Liu: "Pyrethroid Insecticide Cypermethrin Modulates Gonadotropin Synthesis via Calcium Homeostasis and ERK1/2 Signaling in LβT2 Mouse Pituitary Cells." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 162, Issue 1, pp. 43-52, (2019) (PubMed).

Fu, Chen, Xu, Ji, Zhang, Wang, Yu, Xu: "Vitamin D deficiency impairs testicular development and spermatogenesis in mice." dans: Reproductive toxicology (Elmsford, N.Y.), Vol. 73, pp. 241-249, (2018) (PubMed).

Ding, Tan, Song, Ma, Xiao, Zhang: "Effect of controlled ovarian hyperstimulation on puberty and estrus in mice offspring." dans: Reproduction (Cambridge, England), Vol. 154, Issue 4, pp. 433-444, (2018) (PubMed).

Wang, Wang, Lu, Cui, Li, Li, Zhang, Zhang, Liu: "The Combination of icariin and constrained dynamic loading stimulation attenuates bone loss in ovariectomy-induced osteoporotic mice." dans: Journal of orthopaedic research : official publication of the Orthopaedic Research Society, Vol. 36, Issue 5, pp. 1415-1424, (2018) (PubMed).

Ye, Li, Zhang, Ma, Ji, Huang, Curry, Liu, Liu: "Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis." dans: Environmental science & technology, Vol. 51, Issue 17, pp. 10212-10221, (2018) (PubMed).

Cao, Hua, Xiong, Zhu, Zhang, Chen: "Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice." dans: Frontiers in molecular neuroscience, Vol. 11, pp. 6, (2018) (PubMed).

Tang, Yin, Zhang, Chen, Jin, Liu: "Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population." dans: Environmental pollution (Barking, Essex : 1987), Vol. 237, pp. 581-591, (2018) (PubMed).

Varamini, Mansfeld, Giddam, Steyn, Toth: "New gonadotropin-releasing hormone glycolipids with direct antiproliferative activity and gonadotropin-releasing potency." dans: International journal of pharmaceutics, Vol. 521, Issue 1-2, pp. 327-336, (2017) (PubMed).

Miura, Ohtani, Hasegawa, Yoshioka, Hwang: "High sensitivity of testicular function to titanium nanoparticles." dans: The Journal of toxicological sciences, Vol. 42, Issue 3, pp. 359-366, (2017) (PubMed).

Iizuka-Hishikawa, Hishikawa, Sasaki, Takubo, Goto, Nagata, Nakanishi, Shindou, Okamura, Ito, Toshimori, Sasaki, Shimizu: "Lysophosphatidic acid acyltransferase 3 tunes the membrane status of germ cells by incorporating docosahexaenoic acid during spermatogenesis." dans: The Journal of biological chemistry, Vol. 292, Issue 29, pp. 12065-12076, (2017) (PubMed).

Varamini, Rafiee, Giddam, Mansfeld, Steyn, Toth: "Development of New Gonadotropin-Releasing Hormone-Modified Dendrimer Platforms with Direct Antiproliferative and Gonadotropin Releasing Activity." dans: Journal of medicinal chemistry, Vol. 60, Issue 20, pp. 8309-8320, (2017) (PubMed).

Chen, Chen, Tsai, Tzai, Chen, Tsai: "Transdermal Delivery of Luteinizing Hormone-releasing Hormone with Chitosan Microneedles: A Promising Tool for Androgen Deprivation Therapy." dans: Anticancer research, Vol. 37, Issue 12, pp. 6791-6797, (2017) (PubMed).

Liu, Su, Hao, Chen, Liu, Liao, Li: "Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice." dans: International journal of molecular sciences, Vol. 17, Issue 1, (2016) (PubMed).

Spilker, Nullmeier, Grochowska, Schumacher, Butnaru, Macharadze, Gomes, Yuanxiang, Bayraktar, Rodenstein, Geiseler, Kolodziej, Lopez-Rojas, Montag, Angenstein, Bär, DHanis, Roskoden, Mikhaylova et al.: "A Jacob/Nsmf Gene Knockout Results in Hippocampal Dysplasia and Impaired BDNF Signaling in Dendritogenesis. ..." dans: PLoS genetics, Vol. 12, Issue 3, pp. e1005907, (2016) (PubMed).

Feng, Cao, Zhao, Wang, Hua, Chen, Chen: "Exposure of Pregnant Mice to Perfluorobutanesulfonate Causes Hypothyroxinemia and Developmental Abnormalities in Female Offspring." dans: Toxicological sciences : an official journal of the Society of Toxicology, Vol. 155, Issue 2, pp. 409-419, (2016) (PubMed).

Gentilin, Molè, Gagliano, Minoia, Ambrosio, Degli Uberti, Zatelli: "Inhibitory effects of mitotane on viability and secretory activity in mouse gonadotroph cell lines." dans: Reproductive toxicology (Elmsford, N.Y.), Vol. 45, pp. 71-6, (2014) (PubMed).

Okada, Miyake, Yamaguchi, Chiba, Maeta, Bilasy, Edamatsu, Kataoka, Fujisawa: "Critical function of RA-GEF-2/Rapgef6, a guanine nucleotide exchange factor for Rap1, in mouse spermatogenesis." dans: Biochemical and biophysical research communications, Vol. 445, Issue 1, pp. 89-94, (2014) (PubMed).

Liu, Tao, Lu, Yang, Ma, Zhang: "Targeted disruption of the mouse testis-enriched gene Znf230 does not affect spermatogenesis or fertility." dans: Genetics and molecular biology, Vol. 37, Issue 4, pp. 708-15, (2014) (PubMed).

Al-Asmakh, Stukenborg, Reda, Anuar, Strand, Hedin, Pettersson, Söder: "The gut microbiota and developmental programming of the testis in mice." dans: PLoS ONE, Vol. 9, Issue 8, pp. e103809, (2014) (PubMed).

Détail du antigène

Antigène: Luteinizing Hormone (LH)

Abstract: LH Produits

Synonymes: Plod Kit ELISA, procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1 Kit ELISA, Plod1 Kit ELISA

Classe de substances: Hormone