Kallikrein 6 Kit ELISA

N° du produit ABIN6719698

Détail du produit Kallikrein 6 Kit ELISA

Antigène: Kallikrein 6 (KLK6)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 78 pg/mL - 5000 pg/mL

Seuil minimal de détection: 78 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Kallikrein-6, Klk6, KLK6_HUMAN, Neurosin, Protease M, Serine protease 18, Serine protease 9, SP59, Zyme

Background: KLK6(Kallikrein-related peptidase 6), also called KALLIKREIN 6, NEUROSIN, PROTEASE M, ZYME or PRSS9, is a protein that in humans is encoded by the KLK6 gene. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. Northern blot analysis revealed that the PRSS9 mRNA was expressed in several primary tumors and cell lines from mammary, prostate, and ovarian cancers, but was not detected in any metastases of these cancers. The KLK6 gene is mapped on 19q13.41. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Upon cellular stress, neurosin was released from mitochondria to the cytosol, which resulted in the increase of degraded alpha-synuclein species. Neurosin may play a significant role in physiologic alpha-synuclein degradation and also in the pathogenesis of synucleinopathies.

Gene Name: KLK6

Production: Natural and recombinant human Kallikrein-6. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L22-K244

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL, 78pg/mL human Kallikrein-6 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, cell lysates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. We recommend that each human Kallikrein-6 standard solution and each sample is measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 78pg/mL-5000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Kallikrein 6 (KLK6)

Autre désignation: Kallikrein-6 (KLK6 Produits)

Synonymes: Bssp Kit ELISA, Klk7 Kit ELISA, PRSS18 Kit ELISA, PRSS9 Kit ELISA, SP59 Kit ELISA, hK6 Kit ELISA, Prss9 Kit ELISA, AI849898 Kit ELISA, BSP Kit ELISA, Klk29 Kit ELISA, MSP Kit ELISA, Prss18 Kit ELISA, neurosin Kit ELISA, kallikrein related peptidase 6 Kit ELISA, kallikrein related-peptidase 6 Kit ELISA, kallikrein-6 Kit ELISA, KLK6 Kit ELISA, Klk6 Kit ELISA, LOC100716801 Kit ELISA

ID gène: 5653

NCBI Accession: NP_001012982

UniProt: Q92876

Pathways: Système du Complément, Regulation of G-Protein Coupled Receptor Protein Signaling