Thrombopoietin Kit ELISA

N° du produit ABIN6719709

Détail du produit Thrombopoietin Kit ELISA

Antigène: Thrombopoietin (THPO)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 31.2 pg/mL - 2000 pg/mL

Seuil minimal de détection: 31.2 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: C mpl ligand, Megakaryocyte colony stimulating factor, Megakaryocyte growth and development factor, Megakaryocyte stimulating factor, MGC164194, MGDF, MKCSF, ML, MPL ligand, MPLLG, Myeloproliferative leukemia virus oncogene ligand, OTTHUMP00000210504, THPO, TPO

Background: Thrombopoietin(TPO) is also called megakaryocyte growth and development factor(MGDF). TPO, a lineage-specific cytokine affecting the proliferation and maturation of megakaryocytes from committed progenitor cells, is believed to be the major physiological regulator of circulating platelet levels. Human, dog, and mouse cDNAs for MGDF are highly conserved and encode open reading frames for proteins of 353, 352, and 356 amino acids, respectively, including predicted signal peptides. MGDF is a novel cytokine that regulates megakaryocyte development and is a ligand for the MPL receptor. The human MGDF gene has been mapped to chromosome 3q26.3. The standard product used in this kit is gene recombinant mouse TPO, consisting of 419 amino acids with the molecular mass of 35KDa. As a result of glycosylation, the molecular mass is 75KDa.

Gene Name: TPO

Production: Natural and recombinant mouse TPO. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: S22-T356

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse TPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse ell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse TPO standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 31.2pg/mL-2000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Thrombopoietin (THPO)

Autre désignation: Thrombopoietin (TPO) (THPO Produits)

Synonymes: MGDF Kit ELISA, MKCSF Kit ELISA, ML Kit ELISA, MPLLG Kit ELISA, THCYT1 Kit ELISA, TPO Kit ELISA, Mgdf Kit ELISA, Ml Kit ELISA, Mpllg Kit ELISA, Tpo Kit ELISA, Tpo1 Kit ELISA, Tpo2 Kit ELISA, Tpo3 Kit ELISA, Tpo4 Kit ELISA, tpo Kit ELISA, LOC100231762 Kit ELISA, thrombopoietin Kit ELISA, THPO Kit ELISA, Thpo Kit ELISA, thpo Kit ELISA

ID gène: 21832

NCBI Accession: NP_001166976

UniProt: P40226

Pathways: Signalistation JAK/STAT, Hormone Activity