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GDNF Kit ELISA

GDNF Reactivité: Rat Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
N° du produit ABIN6719716
  • Antigène Voir toutes GDNF Kits ELISA
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Reactivité
    • 11
    • 9
    • 8
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    Fonction
    Sandwich ELISA for Quantitative Detection of Antigen
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
    Analytical Method
    Quantitative
    Attributs du produit

    Synonyms: Astrocyte derived trophic factor, ATF, ATF1, ATF2, Glial cell line derived neurotrophic factor, HFB1 GDNF, hGDNF, HSCR4

    Background: Glial cell line-derived neurotrophic factor(GDNF) is a glycosylated, disulfide-bonded homodimer that is a distantly related member of the transforming growth factor-beta superfamily. GDNF, is a potent neurotrophic factor that promotes the survival of dopaminergic neurones in cultures including embryonic neuronal cultures. GDNF, in addition to its potential role in the differentiation and survival of central nervous system neurons, has profound effects on kidney organogenesis and the development of the peripheral nervous system.3 GDNF may have utility in the treatment of Parkinson's disease, which is marked by progressive degeneration of midbrain dopaminergic neurons. GDNF lies on the short arm of human chromosome 5, at 5p13.1-p13.3 ability to promote dopamine uptake in midbrain cultures. The standard product used in this kit is recombinant rat GDNF, which is a dimmer composed of two chains with 134 amino acids.

    Gene Name: GDNF

    Production: Natural and recombinant rat GDNF. There is no detectable cross-reactivity with other relevant proteins.

    Standard: Expression system for standard: sf21, Immunogen sequence: S78-I211

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  • Indications d'application

    Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL rat GDNF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each rat GDNF standard solution and each sample is measured in duplicate.

    Blood Product Anticoagulant: Heparin Sodium

    ELISA Dilution: 31.2pg/mL-2000pg/mL

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    RT,4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
  • Antigène Voir toutes GDNF Kits ELISA
    GDNF (Glial Cell Line Derived Neurotrophic Factor (GDNF))
    Autre désignation
    GDNF (GDNF Produits)
    Synonymes
    gdnf Kit ELISA, GDNF Kit ELISA, AI385739 Kit ELISA, gdnfa Kit ELISA, hfb1-gdnf Kit ELISA, ATF1 Kit ELISA, ATF2 Kit ELISA, HFB1-GDNF Kit ELISA, HSCR3 Kit ELISA, glial cell derived neurotrophic factor Kit ELISA, glial cell derived neurotrophic factor a Kit ELISA, glial cell line derived neurotrophic factor Kit ELISA, glial cell derived neurotrophic factor L homeolog Kit ELISA, Gdnf Kit ELISA, gdnfa Kit ELISA, GDNF Kit ELISA, gdnf.L Kit ELISA
    ID gène
    25453
    NCBI Accession
    NP_062012
    UniProt
    A7UGJ1
    Pathways
    Signalisation RTK, Synaptic Membrane, Tube Formation, Autophagy, Smooth Muscle Cell Migration
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