CCL20 Kit ELISA

N° du produit ABIN6719733

Détail du produit CCL20 Kit ELISA

Antigène: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Beta chemokine exodus 1, Beta-chemokine exodus-1, C C motif chemokine 20, CC chemokine LARC, Ccl20, CCL20(2-70), CCL20_HUMAN, Chemokine (C C motif) ligand 20, CKb4, Exodus 1, LARC, Liver and activation regulated chemokine, Liver and activation-regulated chemokine, Macrophage inflammatory protein 3 alpha, MIP 3 alpha, MIP 3a, MIP-3-alpha, MIP3 alpha, MIP3A, MIP3alpha, SCYA20, Small inducible cytokine A20, Small inducible cytokine subfamily A (Cys Cys) member 20, Small-inducible cytokine A20, ST38

Background: Macrophage Inflammatory Protein 3alpha(MIP3alpha), also called Chemokine, cc motif, ligand 20(CCL20). The MIP-3alpha/CCL20 gene was cloned and sequenced, revealing a four exon, three intron structure, and was localized by FISH analysis to 2q35-q36. MIP3alpha is predominantly expressed in lymph nodes, appendix, PBL, fetal liver, fetal lung and several cell lines. MIP3alpha/CCL20 and its receptor CCR6 are markedly up-regulated in psoriasis, and they may play a role in the recruitment of T cells to lesional psoriatic skin. And Alanine MIP-3alpha and Serine MIP-3alpha, the two forms of MIP3alpha, that differ by one amino acid at the predicted signal peptide cleavage site. Both of them were chemically synthesized and tested for biological activity. And both flu antigen plus IL-2-activated CD4(+) and CD8(+) T lymphoblasts and cord blood-derived dendritic cells responded to Ser and Ala MIP-3alpha.

Gene Name: CCL20

Production: Natural and recombinant human MIP-3 alpha. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence:  A27-M96

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human MIP-3 alpha standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human MIP-3 alpha standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CCL20 (Chemokine (C-C Motif) Ligand 20 (CCL20))

Autre désignation: MIP-3 alpha - CCL20 (CCL20 Produits)

Synonymes: MIP-3ALPHA Kit ELISA, CCL20 Kit ELISA, si:dkey-150o13.1 Kit ELISA, CKb4 Kit ELISA, LARC Kit ELISA, MIP-3-alpha Kit ELISA, MIP-3a Kit ELISA, MIP3A Kit ELISA, SCYA20 Kit ELISA, ST38 Kit ELISA, MIP-3A Kit ELISA, MIP-3[a] Kit ELISA, Scya20 Kit ELISA, exodus-1 Kit ELISA, C-C motif chemokine ligand 20 Kit ELISA, chemokine (C-C motif) ligand 20b Kit ELISA, chemokine (C-C motif) ligand 20 Kit ELISA, CCL20 Kit ELISA, ccl20b Kit ELISA, Ccl20 Kit ELISA

ID gène: 6364

NCBI Accession: NP_001123518

UniProt: P78556

Pathways: Phosphorylation & l'infection par le SRAS-CoV-2