NrCAM Kit ELISA

N° du produit ABIN6719766

Détail du produit NrCAM Kit ELISA

Antigène: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: Bravo, hBravo, Neuronal cell adhesion molecule, Neuronal surface protein Bravo, Ng CAM related, Ng-CAM-related, NgCAM related cell adhesion molecule, NgCAM-related cell adhesion molecule, Nr CAM, Nr-CAM, Nrcam, NRCAM_HUMAN

Background: Neuronal cell adhesion molecule is a protein that in humans is encoded by the NRCAM gene. Cell adhesion molecules (CAMs) are members of the immunoglobulin superfamily. This gene encodes a neuronal cell adhesion molecule with multiple immunoglobulin-like C2-type domains and fibronectin type-III domains. This ankyrin-binding protein is involved in neuron-neuron adhesion and promotes directional signaling during axonal cone growth. Also, this gene is expressed in non-neural tissues and may play a general role in cell-cell communication via signaling from its intracellular domain to the actin cytoskeleton during directional cell migration. Allelic variants of this gene have been associated with autism and addiction vulnerability. Alternative splicing results in multiple transcript variants encoding different isoforms.

Gene Name: NRCAM

Production: Natural and recombinant human NRCAM. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L30-N600

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human NRCAM standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human NRCAM standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: NrCAM (NRCAM) (Neuronal Cell Adhesion Molecule (NRCAM))

Autre désignation: NRCAM (NRCAM Produits)

Synonymes: CD56 Kit ELISA, MSK39 Kit ELISA, NCAM Kit ELISA, Bravo Kit ELISA, C030017F07Rik Kit ELISA, C130076O07Rik Kit ELISA, mKIAA0343 Kit ELISA, NRCAM Kit ELISA, si:dkey-240a12.1 Kit ELISA, Nr-CAM Kit ELISA, neural cell adhesion molecule 1 Kit ELISA, neuronal cell adhesion molecule Kit ELISA, neuronal cell adhesion molecule L homeolog Kit ELISA, neuronal cell adhesion molecule a Kit ELISA, NCAM1 Kit ELISA, NRCAM Kit ELISA, Nrcam Kit ELISA, nrcam.L Kit ELISA, nrcama Kit ELISA, nrcam Kit ELISA

ID gène: 4897

NCBI Accession: NP_001032209

UniProt: Q92823

Pathways: Regulation of Cell Size