Galectin 3 Kit ELISA

N° du produit ABIN6719767

N° du produit (Fournisseur): koa0451

Détail du produit Galectin 3 Kit ELISA

Antigène: Galectin 3 (LGALS3)

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0451

Fournisseur: Rockland

Fonction: Mouse Galectin-3 - LGALS3 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant mouse Galectin-3. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse Galectin-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse Galectin-3 standard solution and each sample be measured in duplicate.

Commentaires:

Standard: Expression system for standard: E.coli, Immunogen sequence: A2-I264

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Détail du antigène

Antigène: Galectin 3 (LGALS3)

Autre désignation: Galectin-3/LGALS3 (LGALS3 Produits)

Sujet:

Synonyms: 36 kDa lectin, Carbohydrate-binding protein 35, CBP 35, Gal-3, Galactose-specific lectin 3, Galactoside-binding protein, GALBP, Galectin-3, GALIG, IgE-binding protein, L-31, Laminin-binding protein, Lectin L-29, lectin, galactoside-binding, soluble, 3, LG_x0000

Background: Galectin-3(GAL3), also known as LGALS3, MAC2 or GALBP, is a member of the lectin family, of which 14 mammalian galectins have been identified. Galectin-3 is encoded by a single gene, LGALS3, located on chromosome 14, locus q21-q22. It is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. Studies have also shown that the expression of galectin-3 is implicated in a variety of processes associated with heart failure, including myofibroblast proliferation, fibrogenesis, tissue repair, inflammation, and Ventricular remodeling. Galectin-3 is expressed in various tissues and organs, but is significantly absent in normal hepatocytes.

ID gène: 16854

NCBI Accession: NP_001139425

UniProt: Q8C253

Pathways: Signalisation RTK