MFGE8 Kit ELISA

N° du produit ABIN6719768

Détail du produit MFGE8 Kit ELISA

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: BA46, Breast epithelial antigen BA46, Contains Medin, EDIL1, HMFG, hP47, HsT19888, Lactadherin, Lactadherin [Precursor], Medin, MFG E8, MFG-E8, MFGE8, MFGM, MFGM_HUMAN, Milk fat globule EGF factor 8, Milk fat globule EGF factor 8 protein, Milk fat globule-EGF factor 8, O acetyl disialoganglioside synthase, OAcGD3S, SED1, SPAG10, Sperm associated antigen 10, Sperm surface protein hP47

Background: MFGE8(Milk Fat Globule-Egf Factor 8), also called as Lactadherin or SED1, is a protein which in humans is encoded by the MFGE8 gene. Mfge8 is secreted protein found in vertebrates, including mammals as well as birds. By fluorescence in situ hybridization, the MFGE8 gene was mapped to chromosome 15q25. MFGE8 is a factor that links apoptotic cells to phagocytes. It specifically bound to apoptotic cells by recognizing aminophospholipids such as phosphatidylserine. MFGE8, when engaged by phospholipids, bound to cells via its RGD motif. It bound particularly strongly to cells expressing alpha-V-beta-3 integrin. It showed that Mfge8 was expressed in intestinal lamina propria macrophages in mice. Using a wound-healing assay, they showed that Mfge8 promoted migration of intestinal epithelial cells through a PKC-epsilon(PRKCE)-dependent mechanism.

Gene Name: MFGE8

Production: Natural and recombinant mouse MFGE8. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A23-C463

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL mouse MFGE8 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse MFGE8 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: MFGE8 (Milk Fat Globule-EGF Factor 8 Protein (MFGE8))

Autre désignation: MFGE8 - Lactadherin (MFGE8 Produits)

Synonymes: BA46 Kit ELISA, EDIL1 Kit ELISA, HMFG Kit ELISA, HsT19888 Kit ELISA, MFG-E8 Kit ELISA, MFGM Kit ELISA, OAcGD3S Kit ELISA, SED1 Kit ELISA, SPAG10 Kit ELISA, hP47 Kit ELISA, ba46 Kit ELISA, edil1 Kit ELISA, oacgd3s Kit ELISA, MGC75581 Kit ELISA, hst19888 Kit ELISA, MGC145109 Kit ELISA, MFGE8 Kit ELISA, mfge8 Kit ELISA, zgc:154157 Kit ELISA, MGC99228 Kit ELISA, AA408458 Kit ELISA, AI325141 Kit ELISA, Mfgm Kit ELISA, P47 Kit ELISA, lactadherin Kit ELISA, AGS Kit ELISA, MFGME8 Kit ELISA, MFGMP-E8 Kit ELISA, im:6895406 Kit ELISA, mfge8l Kit ELISA, zgc:110846 Kit ELISA, milk fat globule-EGF factor 8 protein Kit ELISA, milk fat globule-EGF factor 8 protein b Kit ELISA, milk fat globule-EGF factor 8 protein L homeolog Kit ELISA, milk fat globule-EGF factor 8 protein a Kit ELISA, MFGE8 Kit ELISA, mfge8 Kit ELISA, mfge8b Kit ELISA, mfge8.L Kit ELISA, Mfge8 Kit ELISA, mfge8a Kit ELISA

ID gène: 17304

NCBI Accession: NP_032620

UniProt: P21956

Pathways: SARS-CoV-2 Protein Interactome