CCL21 Kit ELISA

N° du produit ABIN6719772

Détail du produit CCL21 Kit ELISA

Antigène: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Reactivité: Souris

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 15.6 pg/mL - 1000 pg/mL

Seuil minimal de détection: 15.6 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: 6Ckine, Beta chemokine exodus 2, C-C motif chemokine 21, Chemokine (C-C motif) ligand 21, CKb9, ECL, Efficient Chemoattractant for Lymphocytes, Exodus2, MGC34555, OTTHUMP00000000526, OTTHUMP00000000527, OTTHUMP00000021303, SCYA 21, SCYA21, Secondary lymphoid tissue chemokine, SLC, Small inducible cytokine A21, Small inducible cytokine subfamily A (Cys-Cys), member 21, TCA 4, TCA4

Background: Chemokine (C-C motif) ligand 21 (CCL21) is a small cytokine belonging to the CC chemokine family. This chemokine is also known as 6Ckine (because it has six conserved cysteine residues instead of the four cysteines typical to chemokines), exodus-2, and secondary lymphoid-tissue chemokine (SLC). By somatic cell hybrid and radiation hybrid analyses, mapped the SCYA21 gene to 9p13. Chemokines are a family of proteins that direct leukocyte migration and activation to inflammatory stimuli. CXC chemokine ligand 13 (CXCL13), CC chemokine ligand 21 (CCL21), and CCL19 are constitutively expressed in secondary lymphoid organs, where they control the placement of lymphocytes and dendritic cells. It was demonstrate that the local expression of homeostatic chemokines in nonlymphoid organs, such as the lung, plays an important role in protective immune responses.

Gene Name: CCL21

Production: Natural and recombinant mouse CCL21.There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: E.coli, Immunogen sequence: S24-G133

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL mouse CCL21 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each mouse CCL21 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 15.6pg/mL-1000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: CCL21 (Chemokine (C-C Motif) Ligand 21 (CCL21))

Autre désignation: CCL21 - 6Ckine (CCL21 Produits)

Synonymes: 6Ckine Kit ELISA, CKb9 Kit ELISA, ECL Kit ELISA, SCYA21 Kit ELISA, SLC Kit ELISA, TCA4 Kit ELISA, ccl21 Kit ELISA, Ccl21b Kit ELISA, C-C motif chemokine ligand 21 Kit ELISA, C-C motif chemokine 21 Kit ELISA, CCL21 Kit ELISA, ccl21 Kit ELISA, Ccl21 Kit ELISA

ID gène: 18829

NCBI Accession: NP_001180596

UniProt: P84444

Pathways: Regulation of Actin Filament Polymerization