IGFBP5 Kit ELISA

N° du produit ABIN6719773

Détail du produit IGFBP5 Kit ELISA

Antigène: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Plasma (EDTA - heparin), Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: IBP 5, IBP-5, IBP5, IBP5_HUMAN, IGF binding protein 5, IGF BP5, IGF-binding protein 5, IGFBP 5, IGFBP-5, IGFBP5, Insulin like growth factor binding protein 5, Insulin-like growth factor-binding protein 5

Background: Insulin-like growth factor-binding protein 5 is a protein that in humans is encoded by the IGFBP5 gene. The IGFBP5 gene was mapped to chromosome 2q33-q34. The expression of IGFBP5 by stable transfection and adenovirus-mediated infection was inhibitory to growth in 2 human breast cancer cell lines. Stable expression of IGFBP5 in the breast cancer cell lines also inhibited the formation and growth of tumors following injection in athymic mice. IGFBP5 is believed a growth inhibitor and proapoptotic agent in breast cancer cells.

Gene Name: IGFBP5

Production: Natural and recombinant rat IGFBP5. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: L20-E271

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat IGFBP5 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each rat IGFBP5 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: IGFBP5 (Insulin-Like Growth Factor Binding Protein 5 (IGFBP5))

Autre désignation: IGFBP5 (IGFBP5 Produits)

Synonymes: IBP5 Kit ELISA, AI256729 Kit ELISA, AW208790 Kit ELISA, IGFBP-5 Kit ELISA, IGFBP-5P Kit ELISA, IGF-BP5 Kit ELISA, igfbp5 Kit ELISA, ibp5 Kit ELISA, IBP-5 Kit ELISA, xIGFBP-5 Kit ELISA, IGFBP5 Kit ELISA, igfbp5b Kit ELISA, zgc:165472 Kit ELISA, insulin like growth factor binding protein 5 Kit ELISA, insulin-like growth factor binding protein 5 Kit ELISA, insulin like growth factor binding protein 5 L homeolog Kit ELISA, insulin-like growth factor binding protein 5b Kit ELISA, insulin-like growth factor-binding protein 5 Kit ELISA, insulin-like growth factor binding protein 5a Kit ELISA, IGFBP5 Kit ELISA, Igfbp5 Kit ELISA, igfbp5.L Kit ELISA, igfbp5b Kit ELISA, igfbp5 Kit ELISA, LOC100194500 Kit ELISA, igfbp5a Kit ELISA

ID gène: 25285

NCBI Accession: NP_036949

UniProt: P24594

Pathways: Signalisation WNT, Carbohydrate Homeostasis, Myometrial Relaxation and Contraction, Regulation of Carbohydrate Metabolic Process, Autophagy, Smooth Muscle Cell Migration, Growth Factor Binding