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CA9 Kit ELISA

Rockland koa0392 CA9 Reactivité: Humain Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
Rockland
N° du produit ABIN6719792
N° du produit (Fournisseur): koa0392
  • Antigène Voir toutes CA9 Kits ELISA
    CA9 (Carbonic Anhydrase IX (CA9))
    Reactivité
    • 10
    • 6
    • 5
    • 1
    Humain
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    15.6 pg/mL - 1000 pg/mL
    Seuil minimal de détection
    15.6 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0392
    Fournisseur
    Rockland
    Fonction
    Human CA9 Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Supernatant, Plasma (EDTA - heparin - citrate), Serum, Urine
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant human CA9. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 10 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product CA9 Kit ELISA
  • Indications d'application
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL, 15.6pg/mL human CA9 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA, citrate) or urine to each empty well. It is recommended that each human CA9 standard solution and each sample be measured in duplicate.
    Commentaires

    Standard: Expression system for standard: NSO, Immunogen sequence: P59-D414

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Voir toutes CA9 Kits ELISA
    CA9 (Carbonic Anhydrase IX (CA9))
    Autre désignation
    CA9 (CA9 Produits)
    Sujet

    Synonyms: CA-IX, CA9, CAH9_HUMAN, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, Carbonic anhydrase IX, Carbonic dehydratase, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated protein G250, RCC-associated antigen G250, Renal cell carcinoma-associated antigen G250

    Background: Carbonic anhydrase 9(CA9/CAIX) is an enzyme that in humans is encoded by the CA9 gene. Carbonic anhydrases(CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene is mapped to 9p13.3. CA IX is a transmembrane protein and the only tumor-associated carbonic anhydrase isoenzyme known. It is expressed in all clear-cell renal cell carcinoma, but is not detected in normal kidney or most other normal tissues. It may be involved in cell proliferation and transformation.

    ID gène
    768
    NCBI Accession
    NP_001207
    UniProt
    Q16790
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