NGFB Kit ELISA
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- Antigène Voir toutes NGFB Kits ELISA
- NGFB (Nerve Growth Factor beta (NGFB))
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Reactivité
- Souris
- Méthode de détection
- Colorimetric
- Type de méthode
- Sandwich ELISA
- Gamme de detection
- 31.2 pg/mL - 2000 pg/mL
- Seuil minimal de détection
- 31.2 pg/mL
- Application
- ELISA
- N° du produit (Fournisseur)
- koa0277
- Fournisseur
- Rockland
- Fonction
- Mouse NGF - NGF beta Sandwich ELISA Kit for Quantitative Detection
- Marque
- AccuSignal™
- Type d'échantillon
- Cell Culture Supernatant, Serum
- Analytical Method
- Quantitative
- Specificité
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Production: Natural and recombinant mouse NGF. There is no detectable cross-reactivity with other relevant proteins.
- Sensibilité
- < 1 pg/mL
- Ingrédients
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- Antibody-coated 96-well plate
- Target Protein Standard
- Detection antibody
- Detection reagent
- Diluent buffers
- Wash buffers
- Substrate Solution
- Stop solutions
- Adhesive covers
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- Indications d'application
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ELISA_Dilution: 31.2pg/mL-2000pg/mL
Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants or serum to each empty well. It is recommended that each mouse NGF standard solution and each sample be measured in duplicate.
- Commentaires
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Standard: Expression system for standard: E.coli, Immunogen sequence: S122-G241
- Volume d'échantillon
- 100 μL
- Plaque
- Pre-coated
- Restrictions
- For Research Use only
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- Stock
- 4 °C,-20 °C
- Stockage commentaire
- Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
- Date de péremption
- 12 months
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- Antigène Voir toutes NGFB Kits ELISA
- NGFB (Nerve Growth Factor beta (NGFB))
- Autre désignation
- NGF/NGF beta (NGFB Produits)
- Sujet
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Synonyms: Beta nerve growth factor, Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID68
Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.
- ID gène
- 18049
- NCBI Accession
- NP_001106168
- UniProt
- P01139
- Pathways
- Signalisation NF-kappaB, Signalisation RTK, Regulation of Cell Size
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