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NGFB Kit ELISA

Rockland koa0277 NGFB Reactivité: Souris Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Serum
Rockland
N° du produit ABIN6719813
N° du produit (Fournisseur): koa0277
  • Antigène Voir toutes NGFB Kits ELISA
    NGFB (Nerve Growth Factor beta (NGFB))
    Reactivité
    • 7
    • 7
    • 4
    • 1
    Souris
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    31.2 pg/mL - 2000 pg/mL
    Seuil minimal de détection
    31.2 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0277
    Fournisseur
    Rockland
    Fonction
    Mouse NGF - NGF beta Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Supernatant, Serum
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant mouse NGF. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 1 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
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  • Indications d'application

    ELISA_Dilution: 31.2pg/mL-2000pg/mL

    Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 2000pg/mL, 1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.2pg/mL mouse NGF standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants or serum to each empty well. It is recommended that each mouse NGF standard solution and each sample be measured in duplicate.

    Commentaires

    Standard: Expression system for standard: E.coli, Immunogen sequence: S122-G241

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Voir toutes NGFB Kits ELISA
    NGFB (Nerve Growth Factor beta (NGFB))
    Autre désignation
    NGF/NGF beta (NGFB Produits)
    Sujet

    Synonyms: Beta nerve growth factor, Beta NGF, HSAN5, MGC161426, MGC161428, Nerve growth factor (beta polypeptide), Nerve growth factor beta polypeptide, Nerve growth factor beta subunit, NGFB, NID68

    Background: Nerve growth factor(NGF) is a polypeptide involved in the regulation of growth and differentiation of sympathetic and certain sensory neurons. NGF is thought to have a profound effect on the development and maintenance of sympathetic and embryonic sensory neurones. NGF activity isolated from the male mouse submaxillary gland(MSG) consists of three types of subunits, alpha, beta and gamma, which specifically interact to form a 7S, approximately 130,000-molecular weight(Mr) complex. The 7S complex contains two identical 118-amino acid beta-chains, which are solely responsible for the nerve growth-stimulating activity of NGF. NGF, which is expressed by inflammatory cells and effects changes that lead to increased neural responsiveness, could be a pivotal mediator in allergic rhinitis.

    ID gène
    18049
    NCBI Accession
    NP_001106168
    UniProt
    P01139
    Pathways
    Signalisation NF-kappaB, Signalisation RTK, Regulation of Cell Size
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