P-Cadherin Kit ELISA

N° du produit ABIN6719852

Détail du produit P-Cadherin Kit ELISA

Antigène: P-Cadherin (CDH3)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 62.5 pg/mL - 4000 pg/mL

Seuil minimal de détection: 62.5 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Supernatant, Serum

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AI385538, CADH3_HUMAN, Cadherin 3, Cadherin 3 precursor, Cadherin 3 type 1, Cadherin-3, Cadp, Calcium dependent adhesion protein placental, CDH 3, CDH3, CDH3 protein, CDHP, HJMD, P cadherin (placental), P-cadherin, PCAD, Placental cadherin

Background: Cadherins are calcium-dependent cell-cell adhesion molecules that mediate cell-cell binding in a homophilic manner. They play an important role in the growth and development of cells via the mechanisms of control of tissue architecture and the maintenance of tissue integrity. Cadherin expression is regulated spatially as well as temporally. Cadherins are thought to play an important role in development and maintenance of tissues through selective cell-cell adhesion activity and may be involved also in the invasion and metastasis of malignant tumors. Cadherin regulates dendritic spine morphogenesis. A cadherin gene cluster is mapped to a region of chromosome 5 subject to frequent allelic loss in carcinoma. The standard product used in this kit is recombinant P-Cadherin with the molecular mass of 120-130Kda after glycosylation.

Gene Name: CDH3

Production: Natural and recombinant human P-Cadherin. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D108-G654

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 4000pg/mL, 2000pg/mL,1000pg/mL, 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL human P-Cadherin standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates or serum to each empty well. It is recommended that each human P-Cadherin standard solution and each sample be measured in duplicate.

ELISA Dilution: 62.5pg/mL-4000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: P-Cadherin (CDH3)

Autre désignation: P-Cadherin (CDH3 Produits)

Synonymes: CDH3 Kit ELISA, Cadherin-3 Kit ELISA, AI385538 Kit ELISA, Cadp Kit ELISA, Cdhp Kit ELISA, P-cadherin Kit ELISA, Pcad Kit ELISA, CDHP Kit ELISA, HJMD Kit ELISA, PCAD Kit ELISA, E-Cad Kit ELISA, E-cadherin Kit ELISA, EP-cadherin Kit ELISA, XTCAD-1 Kit ELISA, c-cadherin Kit ELISA, cadherin-1 Kit ELISA, cdh1 Kit ELISA, cdh1-b Kit ELISA, cdh3 Kit ELISA, cdhc-A Kit ELISA, cdhp Kit ELISA, hjmd Kit ELISA, l-cam Kit ELISA, lcam Kit ELISA, pcad Kit ELISA, uvo Kit ELISA, uvomorulin Kit ELISA, xb-cad Kit ELISA, xcdh1 Kit ELISA, CDH15 Kit ELISA, K-CAM Kit ELISA, cadherin 3 Kit ELISA, cadherin 3 L homeolog Kit ELISA, Cadherin-3 Kit ELISA, CDH3 Kit ELISA, Cdh3 Kit ELISA, cdh3.L Kit ELISA, cdh-3 Kit ELISA

ID gène: 1001

NCBI Accession: NP_001304124

UniProt: P22223