NRP2 Kit ELISA

N° du produit ABIN6719877

Détail du produit NRP2 Kit ELISA

Antigène: NRP2 (Neuropilin 2 (NRP2))

Reactivité: Rat

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: MGC126574, Neuropilin 2, Neuropilin-2, Neuropilin2, NP 2, NP2, NPN 2, NPN2, NRP 2, Nrp2, NRP2_HUMAN, PRO2714, Receptor for VEGF165 and semaphorins class3, Vascular endothelial cell growth factor 165 receptor 2, Vascular endothelial growth factor 165 receptor 2, VEGF1265R2, VEGF165R2

Background: NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to ...

Gene Name: NRP2

Production: Natural and recombinant rat Neuropilin-2. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: Q23-D857

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Neuropilin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, cell lysates or tissue homogenates to each empty well. It is recommended that each rat Neuropilin-2 standard solution and each sample be measured in duplicate.

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: NRP2 (Neuropilin 2 (NRP2))

Autre désignation: Neuropilin-2 (NRP2 Produits)

Synonymes: NP2 Kit ELISA, NPN2 Kit ELISA, PRO2714 Kit ELISA, VEGF165R2 Kit ELISA, 1110048P06Rik Kit ELISA, Np-2 Kit ELISA, Np2 Kit ELISA, Npn-2 Kit ELISA, Npn2 Kit ELISA, neuropilin-2 Kit ELISA, neuropilin 2 Kit ELISA, NRP2 Kit ELISA, Nrp2 Kit ELISA

ID gène: 81527

NCBI Accession: NP_110496

UniProt: O35276

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals