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NRP2 Kit ELISA

Rockland koa0810 NRP2 Reactivité: Rat Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
Rockland
N° du produit ABIN6719877
N° du produit (Fournisseur): koa0810
  • Antigène Voir toutes NRP2 Kits ELISA
    NRP2 (Neuropilin 2 (NRP2))
    Reactivité
    • 7
    • 5
    • 3
    Rat
    Méthode de détection
    Colorimetric
    Type de méthode
    Sandwich ELISA
    Gamme de detection
    156 pg/mL - 10000 pg/mL
    Seuil minimal de détection
    156 pg/mL
    Application
    ELISA
    N° du produit (Fournisseur)
    koa0810
    Fournisseur
    Rockland
    Fonction
    Rat Neuropilin-2 Sandwich ELISA Kit for Quantitative Detection
    Marque
    AccuSignal™
    Type d'échantillon
    Cell Culture Lysate, Cell Culture Supernatant, Tissue Homogenate
    Analytical Method
    Quantitative
    Specificité

    Production: Natural and recombinant rat Neuropilin-2. There is no detectable cross-reactivity with other relevant proteins.

    Sensibilité
    < 10 pg/mL
    Ingrédients
    • Antibody-coated 96-well plate
    • Target Protein Standard
    • Detection antibody
    • Detection reagent
    • Diluent buffers
    • Wash buffers
    • Substrate Solution
    • Stop solutions
    • Adhesive covers
    Top Product
    Discover our top product NRP2 Kit ELISA
  • Indications d'application
    Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL rat Neuropilin-2 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of rat cell culture supernates, cell lysates or tissue homogenates to each empty well. It is recommended that each rat Neuropilin-2 standard solution and each sample be measured in duplicate.
    Commentaires

    Standard: Expression system for standard: NSO, Immunogen sequence: Q23-D857

    Volume d'échantillon
    100 μL
    Plaque
    Pre-coated
    Restrictions
    For Research Use only
  • Stock
    4 °C,-20 °C
    Stockage commentaire
    Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.
    Date de péremption
    12 months
  • Antigène Voir toutes NRP2 Kits ELISA
    NRP2 (Neuropilin 2 (NRP2))
    Autre désignation
    Neuropilin-2 (NRP2 Produits)
    Sujet

    Synonyms: MGC126574, Neuropilin 2, Neuropilin-2, Neuropilin2, NP 2, NP2, NPN 2, NPN2, NRP 2, Nrp2, NRP2_HUMAN, PRO2714, Receptor for VEGF165 and semaphorins class3, Vascular endothelial cell growth factor 165 receptor 2, Vascular endothelial growth factor 165 receptor 2, VEGF1265R2, VEGF165R2

    Background: NRP2(Neuropilin-2), also called Npn2 or VEGF165R2, encodes a member of the neuropilin family of receptor proteins. The soluble NRP2 was secreted as a 62.5-kD protein following transfection in Chinese hamster ovary cells. The NRP2 gene is mapped to 2q33.3. The NRP2 gene contains 17 exons and spans about 112 kb. This protein may play a role in cardiovascular development, axon guidance, and tumorigenesis. Mice with null mutations in genes encoding Sema3F, and its holoreceptor components Npn2 and plexin A3 (PLEXA3), exhibit increased dentate gyrus granule cell and cortical layer V pyramidal neuron spine number and size, and also aberrant spine distribution. Moreover, Sema3F promotes loss of spines and excitatory synapses in dissociated neurons in vitro, and in NRP2-null brain slices cortical layer V and dentate gyrus granule cells exhibit increased miniature excitatory postsynaptic current frequency. These disparate effects of secreted semaphorins are reflected in the restricted dendritic localization of NRP2 to ...

    ID gène
    81527
    NCBI Accession
    NP_110496
    UniProt
    O35276
    Pathways
    Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
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