CD56 Kit ELISA

N° du produit ABIN6719883

N° du produit (Fournisseur): koa0409

Détail du produit CD56 Kit ELISA

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

N° du produit (Fournisseur): koa0409

Fournisseur: Rockland

Fonction: Human CD56 - NCAM-1 Sandwich ELISA Kit for Quantitative Detection

Marque: AccuSignal™

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Specificité:

Production: Natural and recombinant human CD56. There is no detectable cross-reactivity with other relevant proteins.

Sensibilité: < 10 pg/mL

Ingrédients:

• Antibody-coated 96-well plate
• Target Protein Standard
• Detection antibody
• Detection reagent
• Diluent buffers
• Wash buffers
• Substrate Solution
• Stop solutions
• Adhesive covers

Information d'application

Indications d'application:

ELISA_Dilution: 156 pg/mL-10000 pg/mL

Application_Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human CD56 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. We recommend that each human CD56 standard solution and each sample is measured in duplicate.

Commentaires:

Standard: Expression system for standard: NSO, Immunogen sequence: L20-G718

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: 4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Date de péremption: 12 months

Références pour CD56 Kit ELISA (ABIN6719883)

Jennings, Kagiava, Vendredy, Spaulding, Stavrou, Hathazi, Grüneboom, De Winter, Gess, Schara, Pogoryelova, Lochmüller, Borchers, Roos, Burgess, Timmerman, Kleopa, Horvath: "NCAM1 and GDF15 are biomarkers of Charcot-Marie-Tooth disease in patients and mice." dans: Brain : a journal of neurology, Vol. 145, Issue 11, pp. 3999-4015, (2022) (PubMed).

Détail du antigène

Antigène: CD56 (NCAM1) (Neural Cell Adhesion Molecule 1 (NCAM1))

Autre désignation: CD56/NCAM-1 (NCAM1 Produits)

Sujet:

Synonyms: CD56, N-CAM-1, NCAM-1, NCAM1, NCAM1_HUMAN, Neural cell adhesion molecule 1

Background: Neural Cell Adhesion Molecule, NCAM, also known as the cluster of differentiation CD56, is a hemophilic binding glycoprotein. It is a glycoprotein of Immunoglobulin(Ig) super family. At least 27 alternatively spliced NCAM mRNAs are produced, giving a wide diversity of NCAM isoforms NCAM gene is located at 11q22-q23. This glycoprotein is mainly expressed on the surface of neurons, glia, skeletal muscle and natural killer cells. NCAM has been implicated as having a role in cell-cell adhesion, neurite outgrowth, synaptic plasticity, and learning and memory.

ID gène: 4684

NCBI Accession: NP_000606

UniProt: P13591