Biglycan Kit ELISA

N° du produit ABIN6719885

Détail du produit Biglycan Kit ELISA

Antigène: Biglycan (BGN)

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: BGN, Biglycan, Biglycan proteoglycan, Bone/cartilage proteoglycan I, Dermatan sulphate proteoglycan I, DSPG1, PG S1, PG-S1, PGI, PGS1_HUMAN, SLRR1A, Small leucine rich protein 1A

Background: Biglycan (BGN) is a small leucine-rich repeat proteoglycan (SLRP) which is found in a variety of extracellular matrix tissues, including bone, cartilage and tendon. It is an important structural component of articular cartilage and participates in the assembly of the chondrocyte extracellular matrix through formation of protein interactions with type VI collagen and large proteoglycan aggregates. In humans, Biglycan is encoded by the BGN gene. It is mapped to Xq28. Biglycan is believed to play a role in the mineralisation of bone, and its core protein binds to the growth factors BMP-4 and influences its bioactivity. It has been reported that the presence of biglycan is necessary for BMP-4 to exert its effects on osteoblasts. There is also evidence that biglycan can bind to TGF-beta 1. In addition, Biglycan plays an important role in cell signaling.

Gene Name: BGN

Production: Natural and recombinant human Biglycan. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: D38-K368

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human Biglycan standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum, plasma(heparin, EDTA) or tissue homogenates to each empty well. It is recommended that each human Biglycan standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Other Performance Data: DOE at 4 °C 18JAN19. DOE at -20 °C 19JUL19.

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: Biglycan (BGN)

Autre désignation: Biglycan (BGN Produits)

Synonymes: DSPG1 Kit ELISA, PG-S1 Kit ELISA, PGI Kit ELISA, SLRR1A Kit ELISA, BG Kit ELISA, BSPG1 Kit ELISA, bgn Kit ELISA, zgc:91989 Kit ELISA, dspg1 Kit ELISA, pg-s1 Kit ELISA, slrr1a Kit ELISA, xbgn Kit ELISA, BGN Kit ELISA, zgc:77123 Kit ELISA, DKFZp468I2427 Kit ELISA, biglycan Kit ELISA, biglycan L homeolog Kit ELISA, biglycan a Kit ELISA, biglycan S homeolog Kit ELISA, biglycan b Kit ELISA, BGN Kit ELISA, Bgn Kit ELISA, bgn.L Kit ELISA, bgna Kit ELISA, bgn.S Kit ELISA, bgn Kit ELISA, bgnb Kit ELISA

ID gène: 633

NCBI Accession: NP_001702

UniProt: P21810

Pathways: Glycosaminoglycan Metabolic Process