DKK3 Kit ELISA

N° du produit ABIN6719891

Détail du produit DKK3 Kit ELISA

Antigène: DKK3

Reactivité: Humain

Méthode de détection: Colorimetric

Type de méthode: Sandwich ELISA

Gamme de detection: 156 pg/mL - 10000 pg/mL

Seuil minimal de détection: 156 pg/mL

Application: ELISA

Fonction: Sandwich ELISA for Quantitative Detection of Antigen

Type d'échantillon: Cell Culture Lysate, Cell Culture Supernatant, Plasma (EDTA - heparin), Serum, Tissue Homogenate

Analytical Method: Quantitative

Attributs du produit:

Synonyms: AW061014, C87148, Dickkopf (Xenopus laevis) homolog 3, Dickkopf 3, Dickkopf homolog 3 (Xenopus laevis), Dickkopf related protein 3, HDkk 3, Regulated in glioma, REIC, RIG, RIG like 5 6, RIG like 7 1

Background: Dickkopf-related protein 3 is a protein that in humans is encoded by the DKK3 gene. This gene encodes a protein that is a member of the dickkopf family. It is mapped to 11p15.3. The secreted protein contains two cysteine rich regions and is involved in embryonic development through its interactions with the Wnt signaling pathway. The expression of this gene is decreased in a variety of cancer cell lines and it may function as a tumor suppressor gene. Members of the Dkk-related family display unique patterns of mRNA expression in human and mouse tissues, and are secreted when expressed in 293T cells. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

Gene Name: DKK3

Production: Natural and recombinant human DKK-3. There is no detectable cross-reactivity with other relevant proteins.

Standard: Expression system for standard: NSO, Immunogen sequence: A22-I350

Information d'application

Indications d'application:

Application Note: Useful in Sandwich ELISA for Quantitative Detection of Antigen. Aliquot 0.1 mL per well of the 10,000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL human DKK-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. It is recommended that each human DKK-3 standard solution and each sample be measured in duplicate.

Blood Product Anticoagulant: Heparin Sodium

ELISA Dilution: 156pg/mL-10,000pg/mL

Volume d'échantillon: 100 μL

Plaque: Pre-coated

Restrictions: For Research Use only

Stockage

Stock: RT,4 °C,-20 °C

Stockage commentaire: Store vials at 4°C prior to opening. Centrifuge product if not completely clear after standing at room temperature. This product is stable for 6 months at 4°C as an undiluted liquid. Dilute only prior to immediate use. For extended storage freeze at -20°C or below for 12 months. Avoid cycles of freezing and thawing.

Détail du antigène

Antigène: DKK3

Autre désignation: DKK-3 (DKK3 Produits)

Synonymes: REIC Kit ELISA, RIG Kit ELISA, reic Kit ELISA, dkk-3 Kit ELISA, AW061014 Kit ELISA, C87148 Kit ELISA, Reic Kit ELISA, p29 Kit ELISA, dickkopf WNT signaling pathway inhibitor 3 Kit ELISA, dickkopf WNT signaling pathway inhibitor 3 S homeolog Kit ELISA, DKK3 Kit ELISA, dkk3 Kit ELISA, dkk3.S Kit ELISA, Dkk3 Kit ELISA

ID gène: 27122

NCBI Accession: NP_001018067

UniProt: Q9UBP4